Evidence map›Paper›PMID 36069579›Full record

ArticleApplied and environmental microbiology2022

New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041.

Kosuke Koyama, Yui Mikawa, Shota Nakagawa, Ryota Kurashiki, Takashi Ohshiro, Hirokazu Suzuki

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Efficient pathway-drivenApplied and environmental microbiology · 2024
    Article
  2. Whole-genome sequence ofMicrobiology resource announcements · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kosuke KoyamaDepartment of Engineering, Graduate School of Sustainability Science, Tottori University, Tottori, Japan.
Yui MikawaFaculty of Engineering, Tottori University, Tottori, Japan.
Shota NakagawaFaculty of Engineering, Tottori University, Tottori, Japan.
Ryota KurashikiDepartment of Engineering, Graduate School of Sustainability Science, Tottori University, Tottori, Japan.
Takashi OhshiroFaculty of Engineering, Tottori University, Tottori, Japan.
Hirokazu SuzukiFaculty of Engineering, Tottori University, Tottori, Japan.ORCID 0000-0002-9507-6467
Tottori University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Geobacillus thermodenitrificans K1041 is an unusual thermophile that is highly transformable via electroporation, making it a promising host for screening genetic libraries at elevated temperatures. In this study, we determined its biological properties, draft genome sequence, and effective vectors and also optimized the electroporation procedures in an effort to enhance its utilization. The organism exhibited swarming motility but not detectable endospore formation, and growth was rapid at 60°C under neutral and relatively low-salt conditions. Although the cells showed negligible acceptance of shuttle plasmids from general strains of Escherichia coli, methylation-controlled plasmids from

Indexed as

Escherichia coli ProteinsGeobacillusDNA Restriction EnzymesDNA Restriction-Modification EnzymesEscherichia coliGenetic VectorsGenomicsPlasmidsTemperatureDNA Restriction EnzymesDNA Restriction-Modification EnzymesEscherichia coli ProteinsmcrB protein, E colielectroporationgene expressiongenome sequenceplasmid vectorpromoterprotein productionrestriction-modification systemthermophile

Identifiers

PMID36069579
PMCPMC9499010
OpenAlexW4294920955

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.