ArticleBMC infectious diseases2022
Molecular beacon based real-time PCR p1 gene genotyping, macrolide resistance mutation detection and clinical characteristics analysis of Mycoplasma pneumoniae infections in children.
Article in BMC infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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18 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Global molecular epidemiology ofEmerging microbes & infections · 2026Pooled it
- Reporting macrolide-resistantJournal of clinical microbiology · 2026Review
- Research progress on detection technologies for Mycoplasma pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Clinical features and risk factors of plastic bronchitis in Mycoplasma pneumoniae pneumonia children with pulmonary consolidation: a prospective cohort study.Frontiers in pediatrics · 2026Article
- AgeFrontiers in pediatrics · 2026Article
- Multicenter analysis of the Mycoplasma pneumoniae resurgence in China 2023-2024.Scientific reports · 2025Article
- Serum expression of ESM-1 and Syndecan-1 and its relationship with disease severity in children with Mycoplasma pneumoniae pneumonia.Italian journal of pediatrics · 2025Observational
- Navigating the outbreak: a comprehensive analysis of pediatricMicrobiology spectrum · 2025Article
- C-reactive protein-induced injury inMicrobiology spectrum · 2025Article
- Trends inMicrobiology spectrum · 2025Article
- Epidemiological and clinical characteristics of hospitalized pediatric patients with Mycoplasma pneumoniae pneumonia before and after the COVID-19 pandemic in China: a retrospective multicenter study.BMC infectious diseases · 2025Article
- Advances in adhesion-related pathogenesis inFrontiers in microbiology · 2025Review
- P1 adhesin genotype characteristics ofFrontiers in cellular and infection microbiology · 2025Article
- Whole-genome probe capture sequencing reveals genomic diversity and characteristics ofFrontiers in microbiology · 2025Article
- Epidemiological characteristics of mycoplasma pneumoniae in hospitalized children before, during, and after COVID-19 pandemic restrictions in Chongqing, China.Frontiers in cellular and infection microbiology · 2024Article
- Increased macrolide resistance rate ofFrontiers in microbiology · 2024Article
- Influence of COVID-19 pandemic on the epidemiology ofHeliyon · 2023Article
- Impact of COVID-19 on the Epidemiological Features of Mycoplasma pneumoniae Infection in Children with Community-Acquired Pneumonia in Henan, China.Microbiology spectrum · 2023Article
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10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMycoplasma pneumoniae can be divided into different subtypes on the basis of the sequence differences of adhesive protein P1, but the relationship between different subtypes, macrolide resistance and clinical manifestations are still unclear. In the present study, we established a molecular beacon based real-time polymerase chain reaction (real-time PCR) p1 gene genotyping method, analyzed the macrolide resistance gene mutations and the relationship of clinical characteristics with the genotypes.
methodsA molecular beacon based real-time PCR p1 gene genotyping method was established, the mutation sites of macrolide resistance genes were analyzed by PCR and sequenced, and the relationship of clinical characteristics with the genotypes was analyzed.
resultsThe detection limit was 1-100 copies/reaction. No cross-reactivity was observed in the two subtypes. In total, samples from 100 patients with positive M. pneumoniae detection results in 2019 and 2021 were genotyped using the beacon based real-time PCR method and P1-1 M. pneumoniae accounted for 69.0%. All the patients had the A2063G mutation in the macrolide resistance related 23S rRNA gene. Novel mutations were also found, which were C2622T, C2150A, C2202G and C2443A mutations. The relationship between p1 gene genotyping and the clinical characteristics were not statistically related.
conclusionA rapid and easy clinical application molecular beacon based real-time PCR genotyping method targeting the p1 gene was established. A shift from type 1 to type 2 was found and 100.0% macrolide resistance was detected. Our study provided an efficient method for genotyping M. pneumoniae, valuable epidemiological monitoring information and clinical treatment guidance to control high macrolide resistance.
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