ArticleThe Plant cell2023
BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 84 citations in OpenAlex.
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Optimized tRNA processing and TREX2-SpCas9 fusion enable high-efficiency multiplex genome editing in plants.Plant communications · 2026Article
- Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens.Cell reports · 2026Article
- Evaluation of Transgenerational Gene Editing Efficiency and Inheritance of Edits Using a Split Cas9/gRNA Crossing System in Zea mays.Plant biotechnology journal · 2026Article
- mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.Plant physiology · 2026Article
- Beyond the Edit: Integrating CRISPR-Cas Genome Editing Into the Cereal Breeding Pipeline.International journal of genomics · 2026Review
- A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice.BMC plant biology · 2025Article
- PotatoMASH is a cost-effective marker system for genomic prediction in potato based on short-read haplotypes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- Beautiful and delicious mutants: The origins, fates, and benefits of molecular sequence variation in plant evolution and breeding.Plant physiology · 2025Review
- Comparative transcriptome profiling and co-expression network analysis reveals important genes regulating maize response to Southern corn rust.BMC plant biology · 2025Article
- Complexity Meets Risk-The Next Generation of Genome-Edited Plants Challenges Established Concepts for Environmental Risk Assessment in the EU.Plants (Basel, Switzerland) · 2025Review
- Challenges of translating Arabidopsis insights into crops.The Plant cell · 2025Review
- A magic pocket: An all-in-one CRISPR toolbox for plants.The Plant cell · 2025Article
- Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.Nature communications · 2025Article
- Harnessing haploid-inducer mediated genome editing for accelerated maize variety development.Plant biotechnology journal · 2025Article
- Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR.Plant methods · 2025Article
- dCas-Based Tools to Visualize Chromatin or Modify Epigenetic Marks at Specific Plant Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Embracing native diversity to enhance the maximum quantum efficiency of photosystem II in maize.Plant physiology · 2024Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
Corrections and comments
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- Erratum issued
Authors and funding
17 authors at 1 institution in 1 country.
Funding
Abstract
Ensuring food security for an ever-growing global population while adapting to climate change is the main challenge for agriculture in the 21st century. Although new technologies are being applied to tackle this problem, we are approaching a plateau in crop improvement using conventional breeding. Recent advances in CRISPR/Cas9-mediated gene engineering have paved the way to accelerate plant breeding to meet this increasing demand. However, many traits are governed by multiple small-effect genes operating in complex interactive networks. Here, we present the gene discovery pipeline BREEDIT, which combines multiplex genome editing of whole gene families with crossing schemes to improve complex traits such as yield and drought tolerance. We induced gene knockouts in 48 growth-related genes into maize (Zea mays) using CRISPR/Cas9 and generated a collection of over 1,000 gene-edited plants. The edited populations displayed (on average) 5%-10% increases in leaf length and up to 20% increases in leaf width compared with the controls. For each gene family, edits in subsets of genes could be associated with enhanced traits, allowing us to reduce the gene space to be considered for trait improvement. BREEDIT could be rapidly applied to generate a diverse collection of mutants to identify promising gene modifications for later use in breeding programs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.