ArticleNature communications2022
Molecular basis for polysaccharide recognition and modulated ATP hydrolysis by the O antigen ABC transporter.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Recognition and mechanism of M. tuberculosis cell wall galactan transport.Nature communications · 2026Article
- Structural basis of lipid-linked galactan export by the mycobacterial ABC transporter Wzm-Wzt.Nature communications · 2026Article
- Crystal structure of endo-β-N-acetylglucosaminidase HSα.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Genotype characteristics of two non traveler sources burkholderia pseudomallei strains isolated from clinical patients for the first time in Chongqing, China.BMC infectious diseases · 2025Article
- O-antigen polysaccharides inMicrobiology and molecular biology reviews : MMBR · 2025Review
- A large-scale curated and filterable dataset for cryo-EM foundation model pre-training.Scientific data · 2025Article
- Cryo-EM analyses unveil details of mechanism and targocil-II mediated inhibition of S. aureus WTA transporter TarGH.Nature communications · 2025Article
- The role of ABC transporter DrrABC in the export of PDIM inCell surface (Amsterdam, Netherlands) · 2024Article
- Effects of Cold Plasma Pretreatment on the Synthesis of Polysaccharide from Pleurotus ostreatus.Applied biochemistry and biotechnology · 2024Article
- Article
- Genome wide structural prediction of ABC transporter systems inFrontiers in microbiology · 2024Article
- Architecture of the Heme-translocating CcmABCD/E complex required for Cytochrome c maturation.Nature communications · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
O antigens are ubiquitous protective extensions of lipopolysaccharides in the extracellular leaflet of the Gram-negative outer membrane. Following biosynthesis in the cytosol, the lipid-linked polysaccharide is transported to the periplasm by the WzmWzt ABC transporter. Often, O antigen secretion requires the chemical modification of its elongating terminus, which the transporter recognizes via a carbohydrate-binding domain (CBD). Here, using components from A. aeolicus, we identify the O antigen structure with methylated mannose or rhamnose as its cap. Crystal and cryo electron microscopy structures reveal how WzmWzt recognizes this cap between its carbohydrate and nucleotide-binding domains in a nucleotide-free state. ATP binding induces drastic conformational changes of its CBD, terminating interactions with the O antigen. ATPase assays and site directed mutagenesis reveal reduced hydrolytic activity upon O antigen binding, likely to facilitate polymer loading into the ABC transporter. Our results elucidate critical steps in the recognition and translocation of polysaccharides by ABC transporters.
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