ArticleNature methods2022
Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 73 citations in OpenAlex.
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- Cryogenic STEM of thick biological specimens.Nature methods · 2025Article
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- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Cryo-electron microscopy in color.Nature methods · 2024Article
- Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.Nature methods · 2024Article
- Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods.Viruses · 2024Review
- Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution.Nature communications · 2024Article
- Accurate model and ensemble refinement using cryo-electron microscopy maps and Bayesian inference.PLoS computational biology · 2024Article
- VitroJet: new features and case studies.Acta crystallographica. Section D, Structural biology · 2024Article
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- Imaging built-in electric fields and light matter by Fourier-precession TEM.Scientific reports · 2024Article
- GoldDigger and Checkers, computational developments in cryo-scanning transmission electron tomography to improve the quality of reconstructed volumes.Biological imaging · 2024Article
- Big data in contemporary electron microscopy: challenges and opportunities in data transfer, compute and management.Histochemistry and cell biology · 2023Review
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- Dynamical scattering in ice-embedded proteins in conventional and scanning transmission electron microscopy.IUCrJ · 2023Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC-STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC-STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.
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