ArticleNature communications2022
Structure and activity of particulate methane monooxygenase arrays in methanotrophs.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Quinone-transporting filaments expand bioenergetic capacity in Gram-positive Bacillota.Nature microbiology · 2026Article
- Membrane properties modulate methane oxidation by particulate methane monooxygenase.The Journal of biological chemistry · 2026Article
- HChemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Ammonia monooxygenase: a work in progress.Chemical science · 2026Review
- High-Valent Late Transition Metal-Oxo Complexes: Breaking Boundaries at the Oxo Wall and Beyond.Chemical reviews · 2026Review
- Chemical interference effects with copper-doped mordenite for dilute methane emissions mitigation.RSC advances · 2026Article
- Copper requirements and copper toxicity as niche-defining factors in the growth of terrestrial ammonia-oxidizing archaea and bacteria.FEMS microbiology ecology · 2026Article
- Article
- Knowledge Graph for Methane Selective Conversion: Revisiting and Predicting Product Selectivity and Methane Conversion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Harnessing the potential of microbial methane utilization for chasing sustainability.Current opinion in biotechnology · 2025Review
- In situ cryo-electron microscopy and tomography of cellular and organismal samples.Current opinion in structural biology · 2025Review
- Deciphering the language of mingling lipids and proteins.Current opinion in structural biology · 2025Review
- Direct Methane to Methanol Conversion: An Overview of Non-Syn Gas Catalytic Strategies.Chemical record (New York, N.Y.) · 2025Review
- Structures of methane and ammonia monooxygenases in native membranes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Recent findings in methanotrophs: genetics, molecular ecology, and biopotential.Applied microbiology and biotechnology · 2024Review
- A Review of Polyhydroxyalkanoates: Characterization, Production, and Application from Waste.Microorganisms · 2024Review
- Mechanisms for Methane and Ammonia Oxidation by Particulate Methane Monooxygenase.The journal of physical chemistry. B · 2024Article
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- Computational drug development for membrane protein targets.Nature biotechnology · 2024Review
- EMDB-the Electron Microscopy Data Bank.Nucleic acids research · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
Abstract
Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have potential applications in biomanufacturing. Their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is housed in copper-induced intracytoplasmic membranes (ICMs), of which the function and biogenesis are not known. We show by serial cryo-focused ion beam (cryoFIB) milling/scanning electron microscope (SEM) volume imaging and lamellae-based cellular cryo-electron tomography (cryoET) that these ICMs are derived from the inner cell membrane. The pMMO trimer, resolved by cryoET and subtomogram averaging to 4.8 Å in the ICM, forms higher-order hexagonal arrays in intact cells. Array formation correlates with increased enzymatic activity, highlighting the importance of studying the enzyme in its native environment. These findings also demonstrate the power of cryoET to structurally characterize native membrane enzymes in the cellular context.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.