ArticleMalaria journal2022
Life-history traits of a fluorescent Anopheles arabiensis genetic sexing strain introgressed into South African genomic background.
Article in Malaria journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Development and evaluation of pupal color-based genetic sexing strains in Anastrepha obliqua (Diptera: Tephritidae).Insect science · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Impact of male-sterilizing doses and mating status on blood feeding rates and longevity in Aedes aegypti, Aedes albopictus, and Anopheles arabiensis females.Parasites & vectors · 2026Article
- Advances in male sex separation for the support of mosquito control programs.Frontiers in insect science · 2026Review
- Efficiency assessment of a novel automatic mosquito pupae sex separation system in support of area-wide male-based release strategies.Scientific reports · 2024Article
- Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control.Communications biology · 2023Article
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Abstract
backgroundSouth Africa has set a mandate to eliminate local malaria transmission by 2023. In pursuit of this objective a Sterile Insect Technique programme targeting the main vector Anopheles arabiensis is currently under development. Significant progress has been made towards operationalizing the technology. However, one of the main limitations being faced is the absence of an efficient genetic sexing system. This study is an assessment of an An. arabiensis (AY-2) strain carrying the full Y chromosome from Anopheles gambiae, including a transgenic red fluorescent marker, being introgressed into a South African genetic background as a potential tool for a reliable sexing system.
methodsAdult, virgin males from the An. arabiensis AY-2 strain were outcrossed to virgin females from the South African, Kwazulu-Natal An. arabiensis (KWAG strain) over three generations. Anopheles arabiensis AY-2 fluorescent males were sorted as first instar larvae (L1) using the Complex Object Parametric Analyzer and Sorter (COPAS) and later screened as pupae to verify the sex. Life history traits of the novel hybrid KWAG-AY2 strain were compared to the original fluorescent AY-2 strain, the South African wild-type KWAG strain and a standard laboratory An. arabiensis (Dongola reference strain).
resultsThe genetic stability of the sex-linked fluorescent marker and the integrity and high level of sexing efficiency of the system were confirmed. No recombination events in respect to the fluorescent marker were detected over three rounds of introgression crosses. KWAG-AY2 had higher hatch rates and survival of L1 to pupae and L1 to adult than the founding strains. AY-2 showed faster development time of immature stages and larger adult body size, but lower larval survival rates. Adult KWAG males had significantly higher survival rates. There was no significant difference between the strains in fecundity and proportion of males. KWAG-AY2 males performed better than reference strains in flight ability tests.
conclusionThe life history traits of KWAG-AY2, its rearing efficiency under laboratory conditions, the preservation of the sex-linked fluorescence and perfect sexing efficiency after three rounds of introgression crosses, indicate that it has potential for mass rearing. The potential risks and benefits associated to the use of this strain within the Sterile Insect Technique programme in South Africa are discussed.
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