Evidence map›Paper›PMID 36063325›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezers.

Rachel Leicher, Shixin Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Rachel LeicherLaboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, NY, USA.
Shixin LiuLaboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, NY, USA. shixinliu@rockefeller.edu.
Rockefeller University · USTri-Institutional PhD Program in Chemical Biology · US

Funding

Probing Symmetry Breaking in Epigenetic Inheritance: From Single Molecules to Systems BiologyDP2HG010510 · NHGRI · ROCKEFELLER UNIVERSITY · PI LIU, SHIXIN · 2018 to 2018
$2.5M
NHGRI NIH HHS DP2 HG010510
6 · The paper itself

Abstract

Single-molecule force spectroscopy is a powerful tool to analyze the architecture and interaction of large macromolecular assemblies that are refractory to high-resolution structural interrogations. Here, we describe an optical tweezers-based platform for extracting the mechanical fingerprints of individual nucleosome arrays bound with chromatin-associated complexes, such as the Polycomb repressive complex 2 (PRC2). This platform comprehensively characterizes the diverse binding modes of PRC2 on chromatin, measures their mechanical strengths, and is broadly applicable to the studies of other epigenetic machineries.

Indexed as

ChromatinOptical TweezersNucleosomesPolycomb Repressive Complex 2ChromatinNucleosomesPolycomb Repressive Complex 2Epigenetic machineryNucleosome arrayOptical tweezersPolycomb group proteinsSingle-molecule force spectroscopy

Identifiers

PMID36063325
PMCPMC10751574
OpenAlexW4294577580

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.