ArticleApplied microbiology and biotechnology2022
Lactobacillus casei displaying Clostridium perfringens NetB antigen protects chickens against necrotic enteritis.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Effects of dietary benzoic acid combined with cinnamon essential oil on growth performance, antioxidant capacity, immune function, and gut health in broiler, chickens with necrotic enteritis.Poultry science · 2026Article
- The gut microbiome axis: how Lactobacillus-fermented soymilk orchestrates health.Archives of microbiology · 2026Review
- Antimicrobial and Nutritional Potentials ofInternational journal of molecular sciences · 2025Article
- Dietary Supplementation with Probiotics Alleviates Intestinal Injury in LPS-Challenged Piglets.International journal of molecular sciences · 2025Article
- Oral and parenteral vaccination of broiler chickens with Recombinant NetB antigen from Clostridium perfringens confers significant protection against necrotic enteritis.BMC veterinary research · 2025Article
- Metabolic engineering of Lactobacilli spp. for disease treatment.Microbial cell factories · 2025Review
- Necrotic enteritis and antibiotic-free production of broiler chickens: Challenges in testing and using alternative products.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Article
- A comprehensive review of experimental models and induction protocols for avian necrotic enteritis over the past 2 decades.Frontiers in veterinary science · 2024Review
- A novel bacterium-like particles platform displaying antigens by new anchoring proteins induces efficacious immune responses.Frontiers in microbiology · 2024Article
- Recent Trends on Mitigative Effect of Probiotics on Oxidative-Stress-Induced Gut Dysfunction in Broilers under Necrotic Enteritis Challenge: A Review.Antioxidants (Basel, Switzerland) · 2023Review
- Regulatory Effects of the ProbioticThe journal of poultry science · 2023Review
- Screening of Bacteria InhibitingMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrotic enteritis is a serious economical disease of poultry caused by Clostridium perfringens. NetB toxin of Clostridium perfringens is considered the causative agent of necrotic enteritis. Following the withdrawal of in-feed antibiotic growth promoters, there has been an urgent need to develop alternative approaches such as vaccination. Currently, there are no commercially available vaccines to control necrotic enteritis especially in broiler chickens as the target population. In the present study, we constructed a recombinant Lactobacillus casei strain expressing NetB protein of C. perfringens on the cell surface and used this probiotic-based vaccine strain to immunize broiler chickens orally against experimental induction of necrotic enteritis. The birds immunized with the oral vaccine strain were significantly protected against necrotic enteritis challenge and developed strong serum anti-NetB antibody responses to NetB protein. Furthermore, the immunized birds showed higher body weight gains during the challenge experiment compared with control birds. This study showed, for the first time, that a probiotic-based vector vaccine could be a promising vaccine candidate to provide protection against necrotic enteritis in broiler chickens. KEYPOINTS: • The probiotic L. casei carrying pT1NX-netB plasmid displayed NetB antigen on the cell surface. • The LC-NetB vaccine strain induced high anti-toxin antibody response in broiler chickens. • The LC-NetB vector vaccine provided significant protection against experimental NE challenge.
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