ArticleFrontiers in cellular and infection microbiology2022
A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota.
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Harnessing microfluidics for microbiology: from bacteria-host interactions to emerging cancer therapies.Communications biology · 2026Review
- Microdroplet-based high-throughput screening for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi.Scientific reports · 2026Article
- Microfluidic encapsulation of the human gut microbiota-a tool for research and beyond.Microsystems & nanoengineering · 2026Article
- From diet to function: using synthetic microbial communities to map gut microbial interactions.NPJ biofilms and microbiomes · 2026Review
- Amplicon-based prediction of secondary metabolic potential of microbiomes facilitates natural product discovery.Synthetic and systems biotechnology · 2026Article
- A droplet microfluidic strategy for cultivation, investigation, and high-throughput isolation of mouse gut microbiome bacteria.Applied and environmental microbiology · 2025Article
- Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.Biosensors · 2025Review
- Microfluidics for studying the deep underground biosphere: from applications to fundamentals.FEMS microbiology ecology · 2024Review
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- A Guide to Biodetection in Droplets.Analytical chemistry · 2024Review
- Advances in Culturomics Research on the Human Gut Microbiome: Optimizing Medium Composition and Culture Techniques for Enhanced Microbial Discovery.Journal of microbiology and biotechnology · 2024Review
- Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria.Frontiers in microbiology · 2024Article
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
- Multidimensional specialization and generalization are pervasive in soil prokaryotes.Nature ecology & evolution · 2023Article
- Isolation and Cultivation of Human Gut Microorganisms: A Review.Microorganisms · 2023Review
- A synthetic microbial consortium protects against obesity by regulating vitamin B6 metabolism.Gut microbesArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic interactions within gut microbiota play a vital role in human health and disease. Targeting metabolically interacting bacteria could provide effective treatments; however, obtaining functional bacteria remains a significant challenge due to the complexity of gut microbiota. Here, we developed a facile droplet-based approach to isolate and enrich functional gut bacteria that could utilize metabolites from an engineered butyrate-producing bacteria (EBPB) of anti-obesity potential. This involves the high throughput formation of single-bacteria droplets, followed by culturing "droplets" on agar plates to form discrete single-cell colonies. This approach eliminates the need for sophisticated s instruments to sort droplets and thus allows the operation hosted in a traditional anaerobic chamber. In comparison to the traditional culture, the droplet-based approach obtained a community of substantially higher diversity and evenness. Using the conditioned plates containing metabolites from the EBPB supernatant, we obtained gut bacteria closely associated or interacting with the EBPB. These include anaerobic
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Registered trials
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