Evidence map›Paper›PMID 36061848›Full record

ArticleClinics in orthopedic surgery2022

Feasibility of Loop-Mediated Isothermal Amplification for Rapid Detection of Methicillin-Susceptible and Methicillin-Resistant

Sang-Gyun Kim, Gi Won Choi, Won Seok Choi, Chae Seung Lim, Woong Sik Jang, Ji Hoon Bae

Open access · diamondAbstract read
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Article in Clinics in orthopedic surgery, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Sang-Gyun KimDepartment of Orthopaedic Surgery, National Medical Center, Seoul, Korea.ORCID https://orcid.org/0000-0002-1808-1094
Gi Won ChoiDepartment of Orthopaedic Surgery, Korea University Ansan Hospital, Ansan, Korea.ORCID https://orcid.org/0000-0002-7384-8382
Won Seok ChoiDepartment of Orthopaedic Surgery, Korea University Guro Hospital, Seoul, Korea.ORCID https://orcid.org/0000-0002-7566-6945
Chae Seung LimDepartment of Laboratory Medicine, Korea University Guro Hospital, Seoul, Korea.ORCID https://orcid.org/0000-0002-7765-0840
Woong Sik JangEmergency Medicine, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.ORCID https://orcid.org/0000-0002-2910-0537
Ji Hoon BaeDepartment of Orthopaedic Surgery, Korea University Guro Hospital, Seoul, Korea.ORCID https://orcid.org/0000-0002-6390-0378
Korea University Medical Center · KRKorea University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: To date, few studies have investigated the feasibility of the loop-mediated isothermal amplification (LAMP) assay for identifying pathogens in tissue samples. This study aimed to investigate the feasibility of LAMP for the rapid detection of methicillin-susceptible or methicillin-resistant Methods: Twenty tissue samples infected with either MSSA (n = 10) or MRSA (n = 10) were obtained from patients who underwent orthopedic surgery for suspected musculoskeletal infection between December 2019 and September 2020. DNA was extracted from the infected tissue samples using the bead-beating method. A multiplex LAMP assay was conducted to identify MSSA and MRSA infections. To recognize the Results: The LAMP result was positive for samples containing 10 Conclusions: The results of this study suggest that the LAMP assay performed with tissue DNA samples can be a useful diagnostic method for the rapid detection of musculoskeletal infections caused by MSSA and MRSA.

Indexed as

Methicillin-Resistant Staphylococcus aureusFeasibility StudiesHumansMethicillinMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRNA, Ribosomal, 16SStaphylococcus aureusMethicillinRNA, Ribosomal, 16SLoop-mediated isothermal amplificationMethicillin-resistant Staphylococcus aureusMethicillin-susceptible Staphylococcus aureus

Identifiers

PMID36061848
PMCPMC9393274
OpenAlexW4287854958

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.