ArticleFrontiers in bioengineering and biotechnology2022
Cultivating marine bacteria under laboratory conditions: Overcoming the "unculturable" dogma.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The Biotechnological Applications of Marine Bacteria.Biology · 2026Review
- Screening of Marine Bacteria for Lipase Activity and Application as Whole-Cell Biocatalysts.Microorganisms · 2026Article
- Bioprospecting of the Phylum Bacteroidota for Sustainable Agriculture.Plants (Basel, Switzerland) · 2026Review
- pH stability during fermentation is associated with sustained antibacterial metabolite production in marine sedimentApplied and environmental microbiology · 2026Article
- Dormancy as a Signature of Microbial Community Disassembly during Hydrological Collapse in a Desert Oasis.Microbial ecology · 2026Article
- Metagenomic and Culture-Based Insights into Salinity-Driven Bacterial Community Dynamics throughout Crude Oil-Degrading Enrichment Cultivation.Journal of microbiology and biotechnology · 2026Article
- Exploring the Environmental Resistome and Bacterial Novelty in Marine Isolates from the North Portuguese Coast.Antibiotics (Basel, Switzerland) · 2026Article
- Gems From the Caves: Genomic Insights Into the Biosynthetic Potential of Antimicrobial-Producing Bacteria Associated With Tropical Cave-Dwelling Sponges.Environmental microbiology · 2026Article
- Fungi in the Chilean Altiplano: Analyses of Diversity and Yeasts with Applied Enzymatic Potential.Journal of fungi (Basel, Switzerland) · 2025Article
- Screening and Isolation of Bacterial Strains Able to Degrade Trimethylamine.Microorganisms · 2025Article
- Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.Marine drugs · 2025Review
- Screening strategies and production of biosurfactants (BSs)/bioemulsifiers (BEs) from marine yeasts and fungi.AIMS microbiology · 2025Review
- Impact of growth conditions on the abundance and diversity of cultivable bacteria recovered fromFEMS microbes · 2025Article
- Effects of ciprofloxacin on bacterial abundance and enrichments in samples taken from the sea surface microlayer and underlying waters in the southern North Sea.Frontiers in microbiology · 2025Article
- Uncertainty Analysis of Biogas Generation and Gas Hydrate Accumulations in the Baiyun Sag, South China Sea.Microorganisms · 2024Article
- Impact of salmon farming in the antibiotic resistance and structure of marine bacterial communities from surface seawater of a northern Patagonian area of Chile.Biological research · 2024Article
- Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia.Marine drugs · 2024Review
- Natural Products and Pharmacological Properties of Symbiotic Bacillota (Firmicutes) of Marine Macroalgae.Marine drugs · 2023Review
- The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity.Communications biology · 2023Article
- Bioactivity Profiling and Untargeted Metabolomics of Microbiota Associated with Mesopelagic JellyfishMarine drugs · 2023Article
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2 authors.
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Abstract
Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help the biodiscovery of new products and enzymes from marine bacteria. In this context, we promoted the growth of bacteria from a marine rock pond by culture dependent techniques and compared the results with culture independent methods. The total number of bacteria and diversity were studied in different agar plate media during 6 weeks. Agar plate counting was of the same order of magnitude of direct microscopy counts. The highest efficiency of cultivation was 45% attained in marine agar medium. Molecular analysis revealed 10 different phyla of which only four were isolated by the culture dependent method. On the other hand, four taxonomic orders were detected by cultivation but not by the molecular technique. These include bacteria from the phyla Bacillota and Actinomycetota. Our study shows that it is possible to grow more than the traditionally considered 1% of bacteria from a seawater sample using standard agar plate techniques and laboratorial conditions. The results also demonstrate the importance of culture methods to grow bacteria not detected by molecular approaches for future biotechnological applications.
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