Automatic landmark detection and mapping for 2D/3D registration with BoneNet.

The 3D musculoskeletal motion of animals is of interest for various biological studies and can be derived from X-ray fluoroscopy acquisitions by means of image matching or manual landmark annotation and mapping. While the image matching method requires a robust similarity measure (intensity-based) or an expensive computation (tomographic reconstruction-based), the manual annotation method depends on the experience of operators. In this paper, we tackle these challenges by a strategic approach that consists of two building blocks: an automated 3D landmark extraction technique and a deep neural network for 2D landmarks detection. For 3D landmark extraction, we propose a technique based on the shortest voxel coordinate variance to extract the 3D landmarks from the 3D tomographic reconstruction of an object. For 2D landmark detection, we propose a customized ResNet18-based neural network, BoneNet, to automatically detect geometrical landmarks on X-ray fluoroscopy images. With a deeper network architecture in comparison to the original ResNet18 model, BoneNet can extract and propagate feature vectors for accurate 2D landmark inference. The 3D poses of the animal are then reconstructed by aligning the extracted 2D landmarks from X-ray radiographs and the corresponding 3D landmarks in a 3D object reference model. Our proposed method is validated on X-ray images, simulated from a real piglet hindlimb 3D computed tomography scan and does not require manual annotation of landmark positions. The simulation results show that BoneNet is able to accurately detect the 2D landmarks in simulated, noisy 2D X-ray images, resulting in promising rigid and articulated parameter estimations.