Evidence map›Paper›PMID 36059586›Full record

ArticleSensors and actuators. B, Chemical2022

Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2.

Ting Chen, Pengfei Liu, Huanxiang Wang, Yue Su, Sheng Li, Shimeng Ma, Xuan Xu, Jie Wen, Zhen Zou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sensors and actuators. B, Chemical, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ting ChenLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Pengfei LiuLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Huanxiang WangHunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Chemical Engineering, Changsha University of Science and Technology, Changsha 410114, China.
Yue SuLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Sheng LiLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Shimeng MaLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Xuan XuPeople's Hospital of Hunan Province, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan 410002, China.
Jie WenPeople's Hospital of Hunan Province, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan 410002, China.
Zhen ZouLaboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
Changsha University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great importance to control the COVID-19 pandemic. The gold standard assays for COVID-19 diagnostics are mainly based on separately detecting open reading frame 1ab (ORF1ab) and nucleoprotein (N) genes by RT-PCR. However, the current approaches often obtain false positive-misdiagnose caused by cross-contamination or undesired amplification. To address this issue, herein, we proposed a dumbbell-type triplex molecular switch (DTMS)-based, logic-gated strategy for high-fidelity SARS-CoV-2 RNA detection. The DTMS consists of a triple-helical stem region and two-loop regions for recognizing the ORF1ab and N genes of SARS-CoV-2. Only when the ORF1ab and N gene are concurrent, DTMS experiences a structural rearrangement, thus, bringing the two pyrenes into spacer proximity and leading to a new signal readout. This strategy allows detecting SARS-CoV-2 RNA with a detection limit of 1.3 nM, independent of nucleic acid amplification, holding great potential as an indicator probe for screening of COVID-19 and other population-wide epidemics.

Indexed as

Dumbbell-type triplex molecular switchHigh-fidelityLogic-gateSARS-CoV-2 RNA

Identifiers

PMID36059586
PMCPMC9420052
OpenAlexW4293763363

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.