ArticleRapid communications in mass spectrometry : RCM2022
Improved spatial resolution of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective.
Article in Rapid communications in mass spectrometry : RCM, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Enhancing IR-MALDESI MSI Spatial Resolution Through Beam Constriction With a Ring-Actuated Iris.Rapid communications in mass spectrometry : RCM · 2026Article
- In-source freezing of Trifolium repens leaf tissue to increase spatial resolution and quench stress responses during IR-MALDESI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2026Article
- Ambient high-spatial-resolution mass spectrometry imaging: recent advances in technologies and metabolomics studies.Trends in analytical chemistry : TRAC · 2026Article
- High-Throughput N-Glycan Analysis by IR-MALDESI With a 1.5-kHz Pulsed Mid-IR Laser.Journal of mass spectrometry : JMS · 2026Article
- Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging.Analytical and bioanalytical chemistry · 2025Article
- Comparison of Mid-Infrared and Ultraviolet Lasers Coupled to the MALDESI Source for the Detection of Secondary Metabolites and Structural Lipids in Arabidopsis thaliana.Journal of mass spectrometry : JMS · 2025Article
- Oversampling for Enhanced Spatial Resolution of Zebrafish by Top-Hat IR-MALDESI-MSI.Journal of the American Society for Mass Spectrometry · 2024Article
- Prototyping an ionization source for non-engineers.Journal of mass spectrometry : JMS · 2024Article
- Advances in Imaging Mass Spectrometry for Biomedical and Clinical Research.Trends in analytical chemistry : TRAC · 2023Article
- Evaluating the optimal tissue thickness for mass spectrometry imaging using infrared matrix-assisted laser desorption electrospray ionization.Rapid communications in mass spectrometry : RCM · 2023Article
- High-resolution integrated microfluidic probe for mass spectrometry imaging of biological tissues.Analytica chimica acta · 2023Article
- Maximized Spatial Information and Minimized Acquisition Time of Top-Hat IR-MALDESI-MSI of Zebrafish Using Nested Regions of Interest (nROIs).Journal of the American Society for Mass Spectrometry · 2023Article
- Improved spatial resolution of infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging using a reflective objective.Rapid communications in mass spectrometry : RCM · 2022Article
- Mass spectrometry imaging of N-linked glycans: Fundamentals and recent advances.Mass spectrometry reviewsReview
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4 authors.
Funding
Abstract
rationaleThe level of visual detail of a mass spectrometry image is dependent on the spatial resolution with which it is acquired, which is largely determined by the focal diameter in infrared laser ablation-based techniques. While the use of mid-IR light for mass spectrometry imaging (MSI) has advantages, it results in a relatively large focal diameter and spatial resolution. The continual advancement of infrared matrix-assisted electrospray ionization (IR-MALDESI) for MSI warranted novel methods to decrease laser ablation areas and thus improve spatial resolution.
methodsIn this work, a Schwarzschild-like reflective objective was incorporated into the novel NextGen IR-MALDESI source and characterized on both burn paper and mammalian tissue using an ice matrix. Ablation areas, mass spectra, and annotations obtained using the objective were compared against the current optical train on the NextGen system without modification.
resultsThe effective resolution was determined to be 55 μm by decreasing the step size until oversampling was observed. Use of the objective improved the spatial resolution by a factor of three as compared against the focus lens.
conclusionsA Schwarzschild-like reflective objective was successfully incorporated into the NextGen source and characterized on mammalian tissue using an ice matrix. The corresponding improvement in spatial resolution facilitates the future expansion of IR-MALDESI applications to include those that require fine structural detail.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.