ArticleCell stem cell2022
Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells.
Article in Cell stem cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 79 citations in OpenAlex.
- Placental TRPV2 supports trophoblast function and normal fetal development.Nature communications · 2026Article
- A single small molecule-based human embryo model reveals V-ATPase requirement in mammalian blastocyst cavitation.Cell research · 2026Article
- The embryo game uncovers hidden cell behaviours.Nature communications · 2026Article
- Multi-Omics and Machine Learning Integration Identifies Key Endothelial Modules in Carotid Artery Stenosis.Journal of clinical laboratory analysis · 2026Article
- ESRG maintains human pluripotent stem cell self-renewal by binding NPM1 to regulate BMP4 protein and TGF-β signaling pathway.Cell & bioscience · 2026Article
- Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential.The EMBO journal · 2026Article
- Exiting the ground state: the broad spectrum of cell fates accessible from naïve human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2026Review
- From Stem Cells to Post-gastrulation Amnioids (PGAs): A Protocol to Model Human Extra-Embryonic Development.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optogenetic Rescue Reveals Spatiotemporal Rules of Germ-Layer Patterning.bioRxiv : the preprint server for biology · 2025Article
- Chemical reprogramming of fibroblasts into retinal pigment epithelium cells for vision restoration.Nature communications · 2025Article
- Trophoblast stem cell derivation from naive and primed hPSC enables ELF5 functional analysis.Stem cell reports · 2025Article
- Establishment of human gastrulating stem cells with the capacity of stable differentiation into multiple gastrulating cell types.Cell research · 2025Article
- Single Cell RNA Sequencing and Its Impact on Understanding Human Embryo Development.International journal of molecular sciences · 2025Review
- Juxtaposition of human pluripotent stem cells with amnion-like cells is sufficient to trigger primitive streak formation.bioRxiv : the preprint server for biology · 2025Article
- TFAP2A+ embryonic progenitor cells undergo fate diversification to give rise to human amnion, germline, and mesoderm.bioRxiv : the preprint server for biology · 2025Article
- Integration of single-cell and spatial transcriptomics by SEU-TCA reveals the spatial origin of early cardiac progenitors.Genome biology · 2025Article
- Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs.Nature communications · 2025Article
- α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells.Nature cell biology · 2025Article
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- Stem cell-based models of early human development.Development (Cambridge, England) · 2025Review
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Authors and funding
23 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hallmark of primate postimplantation embryogenesis is the specification of extraembryonic mesoderm (EXM) before gastrulation, in contrast to rodents where this tissue is formed only after gastrulation. Here, we discover that naive human pluripotent stem cells (hPSCs) are competent to differentiate into EXM cells (EXMCs). EXMCs are specified by inhibition of Nodal signaling and GSK3B, are maintained by mTOR and BMP4 signaling activity, and their transcriptome and epigenome closely resemble that of human and monkey embryo EXM. EXMCs are mesenchymal, can arise from an epiblast intermediate, and are capable of self-renewal. Thus, EXMCs arising via primate-specific specification between implantation and gastrulation can be modeled in vitro. We also find that most of the rare off-target cells within human blastoids formed by triple inhibition (Kagawa et al., 2021) correspond to EXMCs. Our study impacts our ability to model and study the molecular mechanisms of early human embryogenesis and related defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.