ArticlePlant biotechnology journal2022
Single-cell RNA-seq reveals fate determination control of an individual fibre cell initiation in cotton (Gossypium hirsutum).
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 1 of them a synthesis that pooled it.
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Who cites it
64 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review of single-cell RNA sequencing applications and insights in Oryza sativa response to environmental stresses.Molecular biology reports · 2025Pooled it
- Trichomes in Plant Defence, Development and Metabolic Integration Under Environmental Stress.Plant, cell & environment · 2026Review
- Multi-organ single-cell transcriptomic atlas identifies QrIAA14 as a candidate negative regulator of adventitious root development in Quercus robur.PLoS genetics · 2026Article
- Multi-Omics Frontiers in Plant Adaptation and Growth: From Molecular Networks to Precision Breeding.Plants (Basel, Switzerland) · 2026Article
- GhNAC47 activates GhSKS6-mediated cell wall remodeling to promote cotton chemical defoliation.The New phytologist · 2026Article
- Systems-level proteomic models of cotton fiber development: a high-resolution data resource to analyze cell dynamics and trait engineering.Plant physiology · 2026Article
- Relief of GbDEAH12Nature communications · 2026Article
- Single-nucleus sequencing and spatial metabolomics analysis reveal the regulatory mechanism of ginkgolic acid biosynthesis in the episperm ofHorticulture research · 2026Article
- Single-cell RNA-sequencing profiles reveal the developmental landscape of hawthorn leaves.BMC plant biology · 2026Article
- Transmembrane Protein GbTMEM209 Inhibits Fibre Elongation via Competitive Interaction With GbHOX3 in Gossypium barbadense.Plant biotechnology journal · 2026Article
- Transcriptional and post-transcriptional regulatory networks controlling cotton fibre development.Plant molecular biology · 2026Review
- Cellular Heterogeneity and Developmental Dynamics of Aril in Papaya.International journal of molecular sciences · 2026Article
- Research on Bamboo Shoot Bud Development: A Leap from Tissue Heterogeneity to Single-Cell Spatial Atlas.Plants (Basel, Switzerland) · 2026Review
- Global atlas of enhancer-promoter interactome in cotton genome revealed by profiling RNA-RNA spatial interactions.Genome biology · 2026Article
- The Genetic Wiring of Plant Trichomes: From Initiation to Fate Specification.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution.Journal of advanced research · 2026Article
- Integrated single-cell transcriptomics and spatial metabolomics unveil cellular differentiation and ginsenosides biosynthesis inHorticulture research · 2025Article
- Duplicated Genes on Homologous Chromosomes Decipher the Dominant Epistasis of the Fiberless Mutant in Cotton.Biology · 2025Article
- Unraveling the Complexity of Plant Trichomes: Models, Mechanisms, and Bioengineering Strategies.International journal of molecular sciences · 2025Review
- Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cotton fibre is a unicellular seed trichome, and lint fibre initials per seed as a factor determines fibre yield. However, the mechanisms controlling fibre initiation from ovule epidermis are not understood well enough. Here, with single-cell RNA sequencing (scRNA-seq), a total of 14 535 cells were identified from cotton ovule outer integument of Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis and the day of anthesis). Three major cell types, fibre, non-fibre epidermis and outer pigment layer were identified and then verified by RNA in situ hybridization. A comparative analysis on scRNA-seq data between Xu142 and its fibreless mutant Xu142 fl further confirmed fibre cluster definition. The developmental trajectory of fibre cell was reconstructed, and fibre cell was identified differentiated at 1 day before anthesis. Gene regulatory networks at four stages revealed the spatiotemporal pattern of core transcription factors, and MYB25-like and HOX3 were demonstrated played key roles as commanders in fibre differentiation and tip-biased diffuse growth respectively. A model for early development of a single fibre cell was proposed here, which sheds light on further deciphering mechanism of plant trichome and the improvement of cotton fibre yield.
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