ArticleeLife2022
Viscoelastic properties of suspended cells measured with shear flow deformation cytometry.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- A microfluidic rheometer for tumor mechanics and invasion studies.Lab on a chip · 2025Article
- A microfluidic rheometer for tumor mechanics and invasion studies.bioRxiv : the preprint server for biology · 2025Article
- Review
- Comprehensive Analysis of Shear Deformation Cytometry Based on Numerical Simulation Method.Biosensors · 2025Article
- Transient flow-induced deformation of cancer cells in microchannels: a general computational model and experiments.Biomechanics and modeling in mechanobiology · 2025Article
- Single-cell electro-mechanical shear flow deformability cytometry.Microsystems & nanoengineering · 2024Article
- Investigations of cardiac fibrosis rheology by in vitro cardiac tissue modeling with 3D cellular spheroids.Journal of the mechanical behavior of biomedical materials · 2024Article
- Single-cell mechanical assay unveils viscoelastic similarities in normal and neoplastic brain cells.Biophysical journal · 2024Article
- Evidence and therapeutic implications of biomechanically regulated immunosurveillance in cancer and other diseases.Nature nanotechnology · 2024Review
- Review on Blood Flow Dynamics in Lab-on-a-Chip Systems: An Engineering Perspective.Chem & bio engineering · 2024Review
- High-throughput single-cell assay for precise measurement of the intrinsic mechanical properties and shape characteristics of red blood cells.Lab on a chip · 2024Article
- Development of a microfluidic cell transfection device into gene-edited CAR T cell manufacturing workflow.Lab on a chip · 2023Article
- Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies.Scientific reports · 2023Article
- Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Viscoelastic properties of suspended cells measured with shear flow deformation cytometry.eLife · 2022Article
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Authors and funding
23 authors.
Funding
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Abstract
Numerous cell functions are accompanied by phenotypic changes in viscoelastic properties, and measuring them can help elucidate higher level cellular functions in health and disease. We present a high-throughput, simple and low-cost microfluidic method for quantitatively measuring the elastic (storage) and viscous (loss) modulus of individual cells. Cells are suspended in a high-viscosity fluid and are pumped with high pressure through a 5.8 cm long and 200 µm wide microfluidic channel. The fluid shear stress induces large, ear ellipsoidal cell deformations. In addition, the flow profile in the channel causes the cells to rotate in a tank-treading manner. From the cell deformation and tank treading frequency, we extract the frequency-dependent viscoelastic cell properties based on a theoretical framework developed by R. Roscoe [1] that describes the deformation of a viscoelastic sphere in a viscous fluid under steady laminar flow. We confirm the accuracy of the method using atomic force microscopy-calibrated polyacrylamide beads and cells. Our measurements demonstrate that suspended cells exhibit power-law, soft glassy rheological behavior that is cell-cycle-dependent and mediated by the physical interplay between the actin filament and intermediate filament networks.
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