ArticleScientific reports2022
Effect of MA01 rhamnolipid on cell viability and expression of quorum-sensing (QS) genes involved in biofilm formation by methicillin-resistant Staphylococcus aureus.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
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- DisarmingPathogens (Basel, Switzerland) · 2025Review
- Bacteriophages Improve the Effectiveness of Rhamnolipids in Combating the Biofilm ofMolecules (Basel, Switzerland) · 2025Article
- Enhancing biofilm disruption and bactericidal efficiency using vancomycin-loaded microbubbles in sonodynamic therapy.JAC-antimicrobial resistance · 2025Article
- Exploring the biofilm inhibitory potential of Candida sp. UFSJ7A glycolipid on siliconized latex catheters.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Production and characterization of the lipopeptide with anti-adhesion for oral biofilm on the surface of titanium for dental implants.Archives of microbiology · 2024Article
- Rutin-coated zinc oxide nanoparticles: a promising antivirulence formulation against pathogenic bacteria.World journal of microbiology & biotechnology · 2024Article
- Proline-Hinged α-Helical Peptides Sensitize Gram-Positive Antibiotics, Expanding Their Physicochemical Properties to Be Used as Gram-Negative Antibiotics.Journal of medicinal chemistry · 2024Article
- Antimycobacterial and Antifungal Activities of Leaf Extracts FromScientifica · 2024Article
- The Synergistic Activity of Rhamnolipid Combined with Linezolid against Linezolid-ResistantMolecules (Basel, Switzerland) · 2023Article
- The great divide: rhamnolipids mediate separation betweenFrontiers in cellular and infection microbiology · 2023Article
- Microbial Biosurfactant as an Alternate to Chemical Surfactants for Application in Cosmetics Industries in Personal and Skin Care Products: A Critical Review.BioMed research international · 2023Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A group of biosurfactants, called rhamnolipids, have been shown to have antibacterial and antibiofilm activity against multidrug-resistant bacteria. Here, we examined the effect of rhamnolipid biosurfactants extracted from Pseudomonas aeruginosa MA01 on cell growth/viability, biofilm formation, and membrane permeability of methicillin-resistant Staphylococcus aureus (MRSA) ATCC6538 bacterial cells. The results obtained from flow cytometry analysis showed that by increasing the concentration of rhamnolipid from 30 to 120 mg/mL, the cell viability decreased by about 70%, and the cell membrane permeability increased by approximately 20%. In fact, increasing rhamnolipid concentration was directly related to cell membrane permeability and inversely related to cell survival. Microtiter plate biofilm assay and laser scanning confocal microscopy analysis revealed that rhamnolipid, at a concentration of 60 mg/mL, exerts a reducing effect on the biofilm formation of Staphylococcus aureus. Real-time PCR analysis for monitoring the relative changes in the expression of agrA, agrC, icaA, and icaD genes involved in biofilm formation and related to the quorum-sensing pathway after treatment with rhamnolipid indicated a reduced expression level of these genes, as well as sortase A gene. The results of the present study deepen our knowledge regarding the use of microbial natural products as promising candidates for therapeutic applications.
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