ArticlePLoS genetics2022
The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Canonical and Variant Nucleosome Reprogramming from Sperm to Blastula.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Turnover of retroelements and satellite DNA drives centromere reorganization over short evolutionary timescales in Drosophila.PLoS biology · 2024Article
- The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila.PLoS genetics · 2022Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
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Abstract
Advances in genomic technology led to a more focused pattern for the distribution of chromosomal proteins and a better understanding of their functions. The recent development of the CUT&RUN technique marks one of the important such advances. Here we develop a modified CUT&RUN technique that we termed nanoCUT&RUN, in which a high affinity nanobody to GFP is used to bring micrococcal nuclease to the binding sites of GFP-tagged chromatin proteins. Subsequent activation of the nuclease cleaves the chromatin, and sequencing of released DNA identifies binding sites. We show that nanoCUT&RUN efficiently produces high quality data for the TRL transcription factor in Drosophila embryos, and distinguishes binding sites specific between two TRL isoforms. We further show that nanoCUT&RUN dissects the distributions of the HipHop and HOAP telomere capping proteins, and uncovers unexpected binding of telomeric proteins at centromeres. nanoCUT&RUN can be readily applied to any system in which a chromatin protein of interest, or its isoforms, carries the GFP tag.
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