Evidence map›Paper›PMID 36047072›Full record

ArticleThe world journal of men's health2023

Analysis of 29 Targeted Genes for Non-Obstructive Azoospermia: The Relationship between Genetic Testing and Testicular Histology.

Rossella Cannarella, Matteo Bertelli, Rosita A Condorelli, Marija Vilaj, Sandro La Vignera, Davor Jezek, Aldo E Calogero

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The world journal of men's health, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Linc00513 sponges miR-7 to modulate TGF-β signaling in azoospermia.European journal of translational myology · 2024
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Rossella CannarellaDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy.ORCID https://orcid.org/0000-0003-4599-8487
Matteo BertelliMAGI Euregio, Bolzano, Italy.ORCID https://orcid.org/0000-0002-9552-221X
Rosita A CondorelliDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy.ORCID https://orcid.org/0000-0002-5217-9343
Marija VilajDepartment of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia.ORCID https://orcid.org/0000-0003-3877-467X
Sandro La VigneraDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy.ORCID https://orcid.org/0000-0002-7113-2372
Davor JezekDepartment of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia.ORCID https://orcid.org/0000-0002-1528-5462
Aldo E CalogeroDepartment of Clinical and Experimental Medicine, University of Catania, Catania, Italy. aldo.calogero@unict.it.ORCID https://orcid.org/0000-0001-6950-335X
University of Catania · ITUniversity Hospital Centre Zagreb · HR

Funding

European Union KK.01.1.1.01.0008Scientific Center of Excellence for Reproductive and Regenerative Medicine
6 · The paper itself

Abstract

purposeTo analyze the presence of potentially pathogenic variants of 29 candidate genes known to cause spermatogenic failure (SPGF) in patients with non-obstructive azoospermia (NOA) who underwent testicular histology. MATERIALS AND

methodsForty-eight patients with unexplained NOA referred to the Department of Transfusion Medicine and Transplantation Biology, University Hospital Center Zagreb, Zagreb, Croatia for testicular biopsy. They were divided into three groups: those who had cryptorchidism (n=9), those with varicocele (n=14), and those with idiopathic NOA (n=25). All included patients underwent blood withdrawal for next-generation sequencing (NGS) analysis and gene sequencing.

resultsWe found a possible genetic cause in 4 patients with idiopathic NOA (16%) and in 2 with cryptorchidism (22%). No pathogenic or possibly pathogenic mutations were identified in patients with varicocele. Variants of undetermined significance (VUS) were found in 11 patients with idiopathic NOA (44%), 3 with cryptorchidism (33%), and 8 patients with varicocele (57%). VUSs of the

conclusionsDifferent mutations of the same gene cause various testicular histological pictures. These results suggest that it is not the gene itself but the type of mutation/variation that determines the testicular histology picture. Based on the data presented above, it remains challenging to design a genetic panel with prognostic value for the outcome of testicular sperm extraction in patients with NOA.

Indexed as

AzoospermiaMale infertilityNext-generation sequencingSpermatogenesisTesticular histology

Identifiers

PMID36047072
PMCPMC10042652
OpenAlexW4286374922

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.