Evidence map›Paper›PMID 36046624›Full record

ArticleCell reports methods2022

Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of

Ceire J Wincott, Gayathri Sritharan, Henry J Benns, Dana May, Carla Gilabert-Carbajo, Monique Bunyan, Aisling R Fairweather, Eduardo Alves, Ivan Andrew, Laurence Game and 4 more

Abstract read
In one paragraph

Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Temporal and spatial dynamics ofProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  6. Article
  7. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Ceire J WincottDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Gayathri SritharanDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Henry J BennsDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Dana MayDepartment of Microbiology, Immunology and Cancer Biology at the Carter Immunology Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
Carla Gilabert-CarbajoDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Monique BunyanHost-Toxoplasma Interaction Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1BF, UK.
Aisling R FairweatherDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Eduardo AlvesDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Ivan AndrewUKRI London Institute of Medical Sciences Genomics Laboratory, Shepherd's Bush, London W12 0NN, UK.
Laurence GameUKRI London Institute of Medical Sciences Genomics Laboratory, Shepherd's Bush, London W12 0NN, UK.
Eva-Maria FrickelHost-Toxoplasma Interaction Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1BF, UK.
Calvin TiengweDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Sarah E EwaldDepartment of Microbiology, Immunology and Cancer Biology at the Carter Immunology Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
Matthew A ChildDepartment of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.

Funding

INFECTIOUS DISEASES TRAINING PROGRAMT32AI007046 · NIAID · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Alison K Criss, William A Petri · 1985 to 2026
$15.3M
Innate Inflammatory Control of CachexiaR35GM138381 · NIGMS · UNIVERSITY OF VIRGINIA · PI Sarah E. Ewald · 2020 to 2026
$2.9M
Cancer Research UKDepartment of HealthNational Centre for the Replacement, Refinement and Reduction of Animals in Research NC/S001239/1NIAID NIH HHS T32 AI007046NIGMS NIH HHS R35 GM138381Wellcome Trust 202553/Z/16/ZWellcome Trust 208780/Z/17/ZWellcome Trust 217202/Z/19/ZWellcome Trust FC001076
6 · The paper itself

Abstract

Cellular barcoding techniques are powerful tools to understand microbial pathogenesis. However, barcoding strategies have not been broadly applied to protozoan parasites, which have unique genomic structures and virulence strategies compared with viral and bacterial pathogens. Here, we present a CRISPR-based method to barcode protozoa, which we successfully apply to

Indexed as

ToxoplasmaAnimalsBrainMiceMice, Inbred CBAProtozoan ProteinsVirulenceProtozoan Proteinscellular barcodingCRISPR-Cas9host-pathogen interactiontissue colonizationToxoplasma gondiiTrypanosoma bruceiwithin-host population dynamics

Identifiers

PMID36046624
PMCPMC9421581

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.