ArticleMicrobiome2022
Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts.
Article in Microbiome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Phenotypic, genomic, and functional characterization of Corynebacterium sp. nov. isolated by droplet-based cultivation from vaginal swabs of a preeclampsia patient.BMC microbiology · 2026Article
- Review
- Harnessing the Gut Microbes of Low Abundance With a Bent-Capillary-Centrifugal-Driven (BCCD) Microdroplet Method.MicrobiologyOpen · 2026Article
- Targeting vaginal dysbiosis: prospects for the application of live biotherapeutics products.Frontiers in microbiology · 2026Review
- Unveiling Neonatal Pneumonia Microbiome by High-throughput Sequencing and Droplet Culturomics.Genomics, proteomics & bioinformatics · 2025Article
- A genomics-based investigation of acetic acid bacteria across a global fermented food metagenomics dataset.iScience · 2025Article
- The Gut Bacteria ofInsects · 2025Article
- Fragmented micro-growth habitats present opportunities for alternative competitive outcomes.Nature communications · 2024Article
- Advances in the isolation, cultivation, and identification of gut microbes.Military Medical Research · 2024Review
- Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis.Microbiome · 2024Article
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSymbiotic gut microbes have a rich genomic and metabolic pool and are closely related to hosts' health. Traditional sequencing profiling masks the genomic and phenotypic diversity among strains from the same species. Innovative droplet-based microfluidic cultivation may help to elucidate the inter-strain interactions. A limited number of bacterial phylotypes colonize the honeybee gut, while individual strains possess unique genomic potential and critical capabilities, which provides a particularly good model for strain-level analyses.
resultsHere, we construct a droplet-based microfluidic platform and generated ~ 6 × 10
conclusionsBy encapsulating single bacteria cells inside microfluidic droplets, we exclude potential interspecific competition for the enrichment of rare strains by shotgun sequencing at high resolution. The comparative genomic analysis reveals underlying mechanisms for host-specific adaptations, providing intriguing insights into microbe-microbe interactions. The current approach may facilitate the hunting for elusive bacteria and paves the way for large-scale studies of more complex animal microbial communities. Video Abstract.
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