ArticleFEMS microbiology letters2022
Enterococcus faecalis V583 cell membrane protein expression to alkaline stress.
Article in FEMS microbiology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 7 citations in OpenAlex.
- Comparative efficacy of semiconductor laser and sodium hypochlorite irrigation for elimination ofFrontiers in medicine · 2026Article
- Effects of Additives on Fermentation Quality, Nutritional Quality, and Microbial Diversity ofMicroorganisms · 2025Article
- The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance ofJournal of oral microbiology · 2025Article
- Genome-wide analysis of acid tolerance genes of Enterococcus faecalis with RNA-seq and Tn-seq.BMC genomics · 2024Article
- Gliotoxin-mediated bacterial growth inhibition is caused by specific metal ion depletion.Scientific reports · 2023Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococcus faecalis is able to adapt to alkaline conditions and is commonly recovered from teeth in which endodontic treatment has failed. The role that E. faecalis membrane proteins play in survival strategies to extreme alkaline conditions is unclear. We grew E. faecalis V583 in a chemostat at pH 8 and 11 at one-tenth the organism's relative maximum growth rate. Following membrane shaving, isotope-coding protein labels were added at the peptide level to samples and then combined. The relative proportion of membrane proteins were identified using LC-ESI mass spectrometry and MaxQuant analysis. Ratios of membrane proteins were log2 transformed, with proteins deviating by more than 1 SD of the mean considered to be up- or down-regulated. A total of six proteins were up-regulated in pH 11 including: EF0669 (polysaccharide biosynthesis family); EF1927 (glycerol uptake facilitator), and EF0114 (glycosyl hydrolase). A total of five proteins were down-regulated including: EF0108 (C4-dicarboxylate transporter); EF1838 (PTS system IIC component); EF0456 (PTS system IID component); and EF0022 (PTS mannose-specific IID component). In extreme alkaline conditions, the membrane proteins of E. faecalis seem to be involved in a shift of carbohydrate metabolism from the PTS system to glycerol, which supports the formation of a protective capsule protecting the cell.
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