ArticleJournal of structural biology2022
Electron-counting MicroED data with the K2 and K3 direct electron detectors.
Article in Journal of structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Characterization of energy filtering slit widths for MicroED data collection.Journal of structural biology: X · 2026Article
- Ultra-rapid cryo-EM data acquisition method enabled by continuous recording based beam image shift.Nature communications · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- A cost-effective 200 kV cryo-EM core facility for high resolution single particle analysis.Methods in microscopy · 2026Article
- Low-Energy Single-Electron Detector with Submicron Resolution.ACS photonics · 2026Article
- Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Limiting the effects of radiation damage in MicroED through dose selection during data processing.Acta crystallographica. Section D, Structural biology · 2025Article
- Auto-thresholding for unbiased electron counting.Microscopy (Oxford, England) · 2025Article
- Fast event-based electron counting for small-molecule structure determination by MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024Article
- Advances and applications of microcrystal electron diffraction (MicroED).Current opinion in structural biology · 2024Review
- Electron counting with direct electron detectors in MicroED.Structure (London, England : 1993) · 2023Article
- Time-resolved electron holography and its application to an ionic liquid specimen.Microscopy (Oxford, England) · 2023Article
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Authors and funding
5 authors.
Funding
Abstract
Microcrystal electron diffraction (MicroED) uses electron cryo-microscopy (cryo-EM) to collect diffraction data from small crystals during continuous rotation of the sample. As a result of advances in hardware as well as methods development, the data quality has continuously improved over the past decade, to the point where even macromolecular structures can be determined ab initio. Detectors suitable for electron diffraction should ideally have fast readout to record data in movie mode, and high sensitivity at low exposure rates to accurately report the intensities. Direct electron detectors are commonly used in cryo-EM imaging for their sensitivity and speed, but despite their availability are generally not used in diffraction. Primary concerns with diffraction experiments are the dynamic range and coincidence loss, which will corrupt the measurement if the flux exceeds the count rate of the detector. Here, we describe instrument setup and low-exposure MicroED data collection in electron-counting mode using K2 and K3 direct electron detectors and show that the integrated intensities can be effectively used to solve structures of two macromolecules between 1.2 Å and 2.8 Å resolution. Even though a beam stop was not used with the K3 studies we did not observe damage to the camera. As these cameras are already available in many cryo-EM facilities, this provides opportunities for users who do not have access to dedicated facilities for MicroED.
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