Evidence map›Paper›PMID 36044843›Full record

ArticleCell reports2022

Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos.

Morteza Roodgar, Fabian P Suchy, Lan H Nguyen, Vivek K Bajpai, Rahul Sinha, Jose G Vilches-Moure, Kevin Van Bortle, Joydeep Bhadury, Ahmed Metwally, Lihua Jiang and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Xenophagocytosis blockade enhances interspecies chimerism.bioRxiv : the preprint server for biology · 2025
    Article
  5. Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025
    Review
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 6 institutions in 2 countries.

Morteza RoodgarDepartment of Genetics, Stanford University, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: mroodgar@stanford.edu.
Fabian P SuchyInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Lan H NguyenInstitute for Computational and Mathematical Engineering, Stanford University, Stanford, CA 94305, USA.
Vivek K BajpaiInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.
Rahul SinhaInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jose G Vilches-MoureDepartment of Comparative Medicine, Stanford University, Stanford, CA 94305, USA.
Kevin Van BortleDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Joydeep BhaduryInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE 413 45 Gothenburg, Sweden.
Ahmed MetwallyDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Lihua JiangDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Ruiqi JianDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Rosaria ChiangDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Angelos OikonomopoulosStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
Joseph C WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
Irving L WeissmanInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
Joseph L MankowskiDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Susan HolmesDepartment of Statistics, Stanford University, Stanford, CA 94305, USA.
Kyle M LohInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Hiromitsu NakauchiDepartment of Genetics, Stanford University, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: nakauchi@stanford.edu.
Catherine A VandeVoortCalifornia National Primate Research Center and Department of Obstetrics and Gynecology, University of California, Davis, Davis, CA, USA. Electronic address: cavandevoort@ucdavis.edu.
Michael P SnyderDepartment of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: mpsnyder@stanford.edu.
Stanford University · USCardiovascular Institute of the South · USStanford Medicine · USJohns Hopkins University · USSahlgrenska University Hospital · SEUniversity of California, Davis · US

Funding

National Institute on Aging (NIA) ColonyP51OD011107 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Simon J. Atkinson · 2012 to 2026
$191.5M
The Southwest National Primate Research Center Supplement- Infrastructure improvements of ABSL2 holding areasP51OD011133 · OD · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI Larry S. Schlesinger · 2012 to 2026
$129.7M
Viral Testing CoreU42OD013117 · OD · JOHNS HOPKINS UNIVERSITY · PI Lydia Hopper · 2017 to 2026
$10.9M
Program in Translational and Experimental HematologyT32HL120824 · NHLBI · STANFORD UNIVERSITY · PI MAJETI, RAVINDRA · 2014 to 2023
$2.4M
Valine as a Metabolic Modulator of HematopoiesisR01DK116944 · NIDDK · STANFORD UNIVERSITY · PI NAKAUCHI, HIROMITSU · 2018 to 2021
$1.5M
Understanding the developmental xenobarrierR01DK121851 · NIDDK · STANFORD UNIVERSITY · PI NAKAUCHI, HIROMITSU · 2020 to 2022
$1.2M
Immunogenomics of susceptibility to tuberculosis (TB) among nonhuman primate speciesK01OD030516 · OD · STANFORD UNIVERSITY · PI Morteza Roodgar · 2022 to 2026
$589k
The development and application of tools to characterize the level and function of RNA polymerase III transcription dynamics during cellular differentiationK99HG010362 · NHGRI · STANFORD UNIVERSITY · PI VAN BORTLE, KEVIN · 2019 to 2020
$238k
NHGRI NIH HHS K99 HG010362NHLBI NIH HHS T32 HL120824NIDDK NIH HHS R01 DK116944NIDDK NIH HHS R01 DK121851NIH HHS K01 OD030516NIH HHS P51 OD011107NIH HHS P51 OD011133NIH HHS U42 OD013117
6 · The paper itself

Abstract

As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.

Indexed as

Induced Pluripotent Stem CellsPan troglodytesAnimalsMacaca mulattaMacaca nemestrinaProteomicsblastocyst, BCL2chimpanzeeCP: Developmental biologycross-species embryosinterspecies chimeraiPSCsNonhuman primatespig-tailed macaque

Identifiers

PMID36044843
PMCPMC10075238
OpenAlexW4293574566

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.