ArticleBioconjugate chemistry2023
Using Spectroscopy to Guide the Adaptation of Aptamers into Electrochemical Aptamer-Based Sensors.
Article in Bioconjugate chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Matrix-Activated Aptamer Sensors for Human Serum Analysis.Angewandte Chemie (International ed. in English) · 2026Article
- Charge-screening dominates signal transduction in electrochemical nucleic acid sensors for aminoglycosides.bioRxiv : the preprint server for biology · 2026Article
- Application of Aptamer-Carbon Surfaces for Electrochemical Label-Free Detection of Vancomycin.Biosensors · 2026Article
- Data-Driven Electrochemistry Reveals the Impact of Hydrophobicity on Aptamer Cross-Reactivity.ACS measurement science au · 2026Article
- Continuous, Week-Long, Seconds-ResolvedJournal of the American Chemical Society · 2026Article
- Article
- On the Design, Fabrication, and Operation of Electrochemical Aptamer-Based Sensors.ACS sensors · 2025Review
- Designing the Future of Biosensing: Advances in Aptamer Discovery, Computational Modeling, and Diagnostic Applications.Biosensors · 2025Review
- Cyclooctyne-Functionalized N-Heterocyclic Carbenes for Click Coupling of Sensing Oligonucleotides to Gold Surfaces.ACS sensors · 2025Article
- Carboxylate-Terminated Electrode Surfaces Improve the Performance of Electrochemical Aptamer-Based Sensors.ACS applied materials & interfaces · 2025Article
- Modular DNA origami-based electrochemical detection of DNA and proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Effects of Physiological-Scale Variation in Cations, pH, and Temperature on the Calibration of Electrochemical Aptamer-Based Sensors.ACS sensors · 2024Article
- Developing Microelectrode Arrays for the Point-of-Care Multiplex Detection of Metabolites.Analytical chemistry · 2024Article
- Codeposition Enhances the Performance of Electrochemical Aptamer-Based Sensors.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Real-Time,ACS sensors · 2022Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Electrochemical aptamer-based (EAB) sensors utilize the binding-induced conformational change of an electrode-attached, redox-reporter-modified aptamer to transduce target recognition into an easily measurable electrochemical output. Because this signal transduction mechanism is single-step and rapidly reversible, EAB sensors support high-frequency, real-time molecular measurements, and because it recapitulates the reagentless, conformation-linked signaling seen in vivo among naturally occurring receptors, EAB sensors are selective enough to work in the complex, time-varying environments found in the living body. The fabrication of EAB sensors, however, requires that their target-recognizing aptamer be modified such that (1) it undergoes the necessary binding-induced conformational change and (2) that the thermodynamics of this "conformational switch" are tuned to ensure that they reflect an acceptable trade-off between affinity and signal gain. That is, even if an "as-selected" aptamer achieves useful affinity and specificity, it may fail when adapted to the EAB platform because it lacks the binding-induced conformational change required to support EAB signaling. In this paper we reveal the spectroscopy-guided approaches we use to modify aptamers such that they support the necessary binding-induced conformational change. Specifically, using newly reported aptamers, we demonstrate the systematic design of EAB sensors achieving clinically and physiologically relevant specificity, limits of detection, and dynamic range against the targets methotrexate and tryptophan.
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Registered trials
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