Evidence map›Paper›PMID 36042885›Full record

ArticleSTAR protocols2022

Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states.

Zijun Chen, Jinqing Huang, Jixi Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Frontiers in microbiology · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Zijun ChenState Key Laboratory of Genetic Engineering, School of Life Sciences and Huashan Hospital, Engineering Research Center of Gene Technology of MOE, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai 200438, China.
Jinqing HuangDepartment of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China.
Jixi LiState Key Laboratory of Genetic Engineering, School of Life Sciences and Huashan Hospital, Engineering Research Center of Gene Technology of MOE, Shanghai Engineering Research Center of Industrial Microorganisms, Fudan University, Shanghai 200438, China. Electronic address: lijixi@fudan.edu.cn.
Fudan University · CNHong Kong University of Science and Technology · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA helicase DDX21 plays vital roles in ribosomal RNA processing and the regulation of host innate immunity during virus infection. Here, we describe the optimized protocols for nucleic acid-free protein purification and crystallization of DDX21 in its different unwinding states. Rational design of the flexible region within the helicase core, and biophysical approach to characterize interactions between DDX21 and RNA, leads to successful crystallization of DDX21. This protocol can be applied to the crystallography of other DExD/H-box RNA helicases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020).

Indexed as

DEAD-box RNA HelicasesRNACrystallizationHumansRNA Processing, Post-TranscriptionalRNA, RibosomalDDX21 protein, humanDEAD-box RNA HelicasesRNARNA, RibosomalMolecular BiologyProtein BiochemistryProtein expression and purificationStructural BiologyX-ray Crystallography

Identifiers

PMID36042885
PMCPMC9420396
OpenAlexW4292384160

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.