ArticleReproductive biology and endocrinology : RB&E2022
MicroRNAs secreted by human preimplantation embryos and IVF outcome.
Article in Reproductive biology and endocrinology : RB&E, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Differentially expressed microRNAs in aneuploid preimplantation blastocysts: a systematic review.Frontiers in reproductive health · 2024Pooled it
- "Old and new" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.Biology of reproduction · 2026Review
- Addressing multiple pregnancies in in vitro fertilization-embryo transfer: Current status, challenges, and solutions.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2026Review
- Effect of preimplantation genetic testing for aneuploidy on pregnancy outcomes of patients with recurrent miscarriage: a retrospective study.BMC pregnancy and childbirth · 2026Article
- Small RNA sequencing in individually selected sperm: Biomarkers for male subfertility and predictors of pregnancy success.Non-coding RNA research · 2026Article
- Analysis of morphological, morphokinetics, cell-free DNA, microRNAs parameters to predict aneuploidy status of embryos.PloS one · 2026Article
- Article
- The role of mitochondrial DNA copy number in spent culture medium in predicting outcomes of single blastocyst transfer.Journal of assisted reproduction and genetics · 2025Article
- Exosomal Communication Between Cumulus-Oocyte Complexes and Granulosa Cells: A New Molecular Axis for Oocyte Competence in Human-Assisted Reproduction.International journal of molecular sciences · 2025Review
- Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation.International journal of molecular sciences · 2025Review
- Comparatively profiling the transcriptome of human, Porcine and mouse oocytes undergoing meiotic maturation.BMC genomics · 2025Article
- Embryo-secreted microRNAs enable non-invasive assessment of chromosomal status and viability inFrontiers in cell and developmental biology · 2025Review
- Overexpression of miR-192 Inhibits In Vitro Porcine Embryo Development by Inducing Oxidative Stress Damage and Impairing Mitochondrial Function.Animals : an open access journal from MDPI · 2024Article
- Investigating the Imperative Role of microRNAs Expression in Human Embryo Implantation: A Narrative Review Based on Recent Evidence.Biomedicines · 2024Review
- A temporal extracellular transcriptome atlas of human pre-implantation development.Cell genomics · 2024Article
- Long-term storage modifies the microRNA expression profile of cryopreserved human semen.Biomolecules & biomedicine · 2024Article
- A validated model for individualized prediction of pregnancy outcome in woman after fresh cycle of Day 5 single blastocyst transfer.Scientific reports · 2023Article
- Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders.International journal of molecular sciences · 2023Article
- Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo generate an effective embryo prediction model and identify a non-invasive evaluation method by analyzing microRNAs (miRNAs) in embryo culture medium.
designAnalysis of microRNA profiles from spent culture medium of blastocysts with good morphology that did or did not result in pregnancy.
settingClinical and experimental research. PATIENTS: Sixty patients who underwent thawed embryo transfer of blastocysts after intracytoplasmic sperm injection. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The association of miRNA abundance levels secreted by blastocysts in culture medium and implantation success.
resultsOur RNA sequencing analysis found a total of 53 differentially expressed miRNAs in the culture media of pregnancy and non-pregnancy groups. Twenty-one miRNAs were analyzed for their potential to predict implantation success. Eight miRNAs (hsa-miR-191-5p, hsa-miR-320a, hsa-miR-92a-3p, hsa-miR-509-3p, hsa-miR-378a-3p, hsa-miR-28-3p, hsa-miR-512-5p, and hsa-miR-181a-5p) were further extracted from the results of a logistic regression analysis of qPCR Ct values. A prediction model for high-quality blastocysts was generated using the eight miRNAs, with an average accuracy of 0.82 by 5-fold cross validation.
conclusionWe isolated blastocyst miRNAs that may predict implantation success and created a model to predict viable embryos. Increasing the number of investigated cases and further studying the effect of each miRNA on embryonic development is needed to refine the miRNA-based predictive model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.