ArticleBMC biotechnology2022
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass.
Article in BMC biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 45 citations in OpenAlex.
- Decoding the Mystery of Sugarcane Genome-Based Breeding: Current Advancements, Strategies, and Future Challenges.Plant, cell & environment · 2026Review
- Multisite Field Evaluation of Oil Accumulation and Agronomic Performance in Grain and Sweet Sorghums Engineered for Lipid Hyperaccumulation.Plant biotechnology journal · 2026Article
- Expression of a Bacterial Trehalose 6-Phosphate Synthase Gene otsA in Camelina sativa Seeds Promotes the Channelling of Carbon Towards Oil Accumulation.Plant biotechnology journal · 2026Article
- An α/β-Hydrolase GmABHD6 Controls Seed Oil Content and Yield in Soybean.Plant biotechnology journal · 2026Article
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Inducible expression of DEFECTIVE IN ANTHER DEHISCENCE 1 enhances triacylglycerol accumulation and lipid droplet formation in vegetative tissues.The Plant journal : for cell and molecular biology · 2025Article
- Development of vegetative oil sorghum: From lab-to-field.Plant biotechnology journal · 2025Article
- Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: 'transgenic oilcane'.Biotechnology for biofuels and bioproducts · 2025Article
- Toward sustainable crops: integrating vegetative (non-seed) lipid storage, carbon-nitrogen dynamics, and redox regulation.Frontiers in plant science · 2025Review
- CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.Functional & integrative genomics · 2024Review
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnology for biofuels and bioproducts · 2023Article
- Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production.Biotechnology for biofuels and bioproducts · 2023Article
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe metabolic engineering of high-biomass crops for lipid production in their vegetative biomass has recently been proposed as a strategy to elevate energy density and lipid yields for biodiesel production. Energycane and sugarcane are highly polyploid, interspecific hybrids between Saccharum officinarum and Saccharum spontaneum that differ in the amount of ancestral contribution to their genomes. This results in greater biomass yield and persistence in energycane, which makes it the preferred target crop for biofuel production.
resultsHere, we report on the hyperaccumulation of triacylglycerol (TAG) in energycane following the overexpression of the lipogenic factors Diacylglycerol acyltransferase1-2 (DGAT1-2) and Oleosin1 (OLE1) in combination with RNAi suppression of SUGAR-DEPENDENT1 (SDP1) and Trigalactosyl diacylglycerol1 (TGD1). TAG accumulated up to 1.52% of leaf dry weight (DW,) a rate that was 30-fold that of non-modified energycane, in addition to almost doubling the total fatty acid content in leaves to 4.42% of its DW. Pearson's correlation analysis showed that the accumulation of TAG had the highest correlation with the expression level of ZmDGAT1-2, followed by the level of RNAi suppression for SDP1.
conclusionsThis is the first report on the metabolic engineering of energycane and demonstrates that this resilient, high-biomass crop is an excellent target for the further optimization of the production of lipids from vegetative tissues.
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