Evidence map›Paper›PMID 36042376›Full record

ArticleScientific reports2022

Localized efficacy of environmental RNAi in Tetranychus urticae.

Nicolas Bensoussan, Maja Milojevic, Kristie Bruinsma, Sameer Dixit, Sean Pham, Vinayak Singh, Vladimir Zhurov, Miodrag Grbić, Vojislava Grbić

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Microscopic Menaces: The Impact of Mites on Human Health.International journal of molecular sciences · 2024
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Nicolas BensoussanDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Maja MilojevicDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Kristie BruinsmaDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Sameer DixitDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Sean PhamDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Vinayak SinghDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Vladimir ZhurovDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Miodrag GrbićDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada.
Vojislava GrbićDepartment of Biology, The University of Western Ontario, 1151 Richmond Street, London, ON, N6A 5B7, Canada. vgrbic@uwo.ca.
Western University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Environmental RNAi has been developed as a tool for reverse genetics studies and is an emerging pest control strategy. The ability of environmental RNAi to efficiently down-regulate the expression of endogenous gene targets assumes efficient uptake of dsRNA and its processing. In addition, its efficiency can be augmented by the systemic spread of RNAi signals. Environmental RNAi is now a well-established tool for the manipulation of gene expression in the chelicerate acari, including the two-spotted spider mite, Tetranychus urticae. Here, we focused on eight single and ubiquitously-expressed genes encoding proteins with essential cellular functions. Application of dsRNAs that specifically target these genes led to whole mite body phenotypes-dark or spotless. These phenotypes were associated with a significant reduction of target gene expression, ranging from 20 to 50%, when assessed at the whole mite level. Histological analysis of mites treated with orally-delivered dsRNAs was used to investigate the spatial range of the effectiveness of environmental RNAi. Although macroscopic changes led to two groups of body phenotypes, silencing of target genes was associated with the distinct cellular phenotypes. We show that regardless of the target gene tested, cells that displayed histological changes were those that are in direct contact with the dsRNA-containing gut lumen, suggesting that the greatest efficiency of the orally-delivered dsRNAs is localized to gut tissues in T. urticae.

Indexed as

TetranychidaeAnimalsPest ControlRNA, Double-StrandedRNA InterferenceRNA, Double-Stranded

Identifiers

PMID36042376
PMCPMC9427735
OpenAlexW4293858607

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.