Evidence map›Paper›PMID 36042257›Full record

ArticleScientific reports2022

NMR analysis suggests the terminal domains of Robo1 remain extended but are rigidified in the presence of heparan sulfate.

Robert V Williams, Chin Huang, Kelley W Moremen, I Jonathan Amster, James H Prestegard

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Robert V WilliamsComplex Carbohydrate Research Center and Department of Chemistry, University of Georgia, Athens, GA, USA.
Chin HuangComplex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Kelley W MoremenComplex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.ORCID 0000-0003-1768-582X
I Jonathan AmsterComplex Carbohydrate Research Center and Department of Chemistry, University of Georgia, Athens, GA, USA.ORCID 0000-0001-7523-5144
James H PrestegardComplex Carbohydrate Research Center and Department of Chemistry, University of Georgia, Athens, GA, USA. jpresteg@ccrc.uga.edu.ORCID 0000-0002-1602-4790
University of Georgia · US

Funding

NMR of Cell Surface OligosaccharidesR01GM033225 · NIGMS · YALE UNIVERSITY · PI PRESTEGARD, JAMES H. · 1985 to 2021
$4.6M
Sparse NMR Labeling Approach to Glycoprotein Structure and FunctionR01GM134335 · NIGMS · UNIVERSITY OF GEORGIA · PI PRESTEGARD, JAMES H. · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM033225NIGMS NIH HHS R01 GM134335NIH HHS R01GM033225
6 · The paper itself

Abstract

Human roundabout 1 (hRobo1) is an extracellular receptor glycoprotein that plays important roles in angiogenesis, organ development, and tumor progression. Interaction between hRobo1 and heparan sulfate (HS) has been shown to be essential for its biological activity. To better understand the effect of HS binding we engineered a lanthanide-binding peptide sequence (Loop) into the Ig2 domain of hRobo1. Native mass spectrometry was used to verify that loop introduction did not inhibit HS binding or conformational changes previously suggested by gas phase ion mobility measurements. NMR experiments measuring long-range pseudocontact shifts were then performed on

Indexed as

Heparan SulfateHumansMagnetic Resonance SpectroscopyNerve Tissue ProteinsReceptors, ImmunologicRoundabout ProteinsSignal TransductionHeparan SulfateNerve Tissue ProteinsReceptors, ImmunologicRoundabout Proteins

Identifiers

PMID36042257
PMCPMC9427851
OpenAlexW4293702667

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.