ArticleNature communications2022
Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Single-monosaccharide resolution glycan profiling enabled by the nanopipette.Chemical science · 2026Article
- Single-Molecule Fingerprinting of Unlabeled Full-Length Proteins Using an Aerolysin Nanopore.Journal of the American Chemical Society · 2026Article
- Enzymatically hydrolyzed oligosaccharide fingerprinting using nanopipettes at the single-molecule level.Chemical science · 2026Article
- A universal deep learning framework for empowering nanopore identification by reinforcing temporal signals.Nature communications · 2026Article
- Coil-Globule versus Field-Driven Coil-Blob Transitions of PEG under Nanopore Confinement.ACS measurement science au · 2026Article
- Glycan Sequencing, A Brief Primer.Glycoscience & therapy · 2026Article
- Review
- Biomimetic bilayer hydrogel coating with antithrombotic and anticalcification properties for cardiovascular tissue engineering application.Regenerative biomaterials · 2026Article
- Single-Molecule Discrimination of Multisialylated Ganglioside Oligosaccharides Using an Engineered Nanopore.Research (Washington, D.C.) · 2026Article
- Dermatan Sulfate: Structure, Biosynthesis, and Biological Roles.Biomolecules · 2025Review
- Vectorial Discrimination of Small Molecules with a Macrocycle Adaptor-Protein Nanopore System and Nanocavity-Dependent, pH Gradient-Controlled Analyte Kinetics.Analytical chemistry · 2025Article
- The Road Toward Nanopore Sequencing of Glycosaminoglycans.Handbook of experimental pharmacology · 2025Review
- Research Progress on Saccharide Molecule Detection Based on Nanopores.Sensors (Basel, Switzerland) · 2024Review
- Nanopore-based glycan sequencing: state of the art and future prospects.Chemical science · 2024Review
- Solid-State Nanopore/Nanochannel Sensing of Single Entities.Topics in current chemistry (Cham) · 2023Review
- Identification of tagged glycans with a protein nanopore.Nature communications · 2023Article
- Glycosaminoglycans: What Remains To Be Deciphered?JACS Au · 2023Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosaminoglycans are highly anionic functional polysaccharides with information content in their structure that plays a major role in the communication between the cell and the extracellular environment. The study presented here reports the label-free detection and analysis of glycosaminoglycan molecules at the single molecule level using sensing by biological nanopore, thus addressing the need to decipher structural information in oligo- and polysaccharide sequences, which remains a major challenge for glycoscience. We demonstrate that a wild-type aerolysin nanopore can detect and characterize glycosaminoglycan oligosaccharides with various sulfate patterns, osidic bonds and epimers of uronic acid residues. Size discrimination of tetra- to icosasaccharides from heparin, chondroitin sulfate and dermatan sulfate was investigated and we show that different contents and distributions of sulfate groups can be detected. Remarkably, differences in α/β anomerization and 1,4/1,3 osidic linkages can also be detected in heparosan and hyaluronic acid, as well as the subtle difference between the glucuronic/iduronic epimers in chondroitin and dermatan sulfate. Although, at this stage, discrimination of each of the constituent units of GAGs is not yet achieved at the single-molecule level, the resolution reached in this study is an essential step toward this ultimate goal.
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