Evidence map›Paper›PMID 36040489›Full record

ArticleApplied microbiology and biotechnology2022

Schultriene and nigtetraene: two sesterterpenes characterized from pathogenetic fungi via genome mining approach.

Lan Jiang, Huanting Yang, Xue Zhang, Xiaoying Li, Kangjie Lv, Weiyan Zhang, Guoliang Zhu, Chengwei Liu, Yongheng Wang, Tom Hsiang and 2 more

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Research Progress on Fungal Sesterterpenoids Biosynthesis.Journal of fungi (Basel, Switzerland) · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Lan JiangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Huanting YangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Xue ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Xiaoying LiState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Kangjie LvState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Weiyan ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Guoliang ZhuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Chengwei LiuKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.
Yongheng WangInstitute of Traditional Chinese Medicine and Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, China.
Tom HsiangSchool of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON, Canada.
Lixin ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China.
Xueting LiuState Key Laboratory of Bioreactor Engineering, East China University of Science of Technology, Shanghai, China. liuxueting@ecust.edu.cn.ORCID http://orcid.org/0000-0002-1322-8253
East China University of Science and Technology · CNJinan University · CNNortheast Forestry University · CNUniversity of Guelph · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fungal bifunctional terpene synthases (BFTSs) have been reported to contribute to the biosynthesis of a variety of di/sesterterpenes via different carbocation transportation pathways. Genome mining of new BFTSs from unique fungal resources will, theoretically, allow for the identification of new terpenes. In this study, we surveyed the distribution of BFTSs in our in-house collection of 430 pathogenetic fungi and preferred two BFTSs (CsSS and NnNS), long distance from previously characterized BFTSs and located in relatively independent branches, based on the established phylogenetic tree. The heterologous expression of the two BFTSs in Aspergillus oryzae and Saccharomyces cerevisiae led to the identification of two new sesterterpenes separately, 5/12/5 tricyclic type-A sesterterpene (schultriene, 1) for CsSS and 5/11 bicyclic type-B sesterterpene (nigtetraene, 2) for NnNS. In addition, to the best of our knowledge, 2 is the first 5/11 bicyclic type-B characterized sesterterpene to date. On the basis of this, the plausible cyclization mechanisms of 1 and 2 were proposed based on density functional theory calculations. These new enzymes and their corresponding terpenes suggest that the chemical spaces produced by BFTSs remain large and also provide important evidences for further protein engineering for new terpenes and for understanding of cyclization mechanism catalyzed by BFTSs. KEY POINTS: • Genome mining of two BFTSs yields two new sesterterpenoids correspondingly. • Identification of the first 5/11 ring system type-B product. • Parse out the rational cyclization mechanism of isolated sesterterpenoids.

Indexed as

Aspergillus oryzaeSesterterpenesCyclizationFungiPhylogenyTerpenesSesterterpenesTerpenesBifunctional terpene synthasesCyclization mechanismsGenome miningHeterologous expressionSesterterpene

Identifiers

PMID36040489
OpenAlexW4293769742

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.