ReviewApplied microbiology and biotechnology2022
Pathway engineering facilitates efficient protein expression in Pichia pastoris.
Review in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Systematic optimization ofEngineering microbiology · 2026Review
- Heterologous expression of hagfish thread proteins EsTKα and EsTKγ inSynthetic and systems biotechnology · 2026Article
- Enhanced biosynthesis of full-length triple-helical human type III collagen in engineered Komagataella phaffii.Applied microbiology and biotechnology · 2026Article
- Advances in Key Genetic Elements and Strategies for High-Yield Heterologous Protein Expression inJournal of fungi (Basel, Switzerland) · 2026Review
- Overcoming prokaryotic toxicity: a SUMO-fused secretory platform in Komagataella phaffii for high-yield production of phage holin Hol41.Microbial cell factories · 2026Article
- Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization.Microbial cell factories · 2026Article
- Strategies for Optimizing the Yield and Function of Recombinant Collagen in Different Expression Systems: A Review.International journal of molecular sciences · 2026Review
- Boosting Recombinant Bovine Chymosin inFoods (Basel, Switzerland) · 2026Article
- A New Paradigm of Bispecific Antibodies in Clinical Management of Gastrointestinal Cancers.Oncology research · 2026Review
- Mechanistic insights into sodium citrate-induced metabolic rewiring for enhanced S-adenosylmethionine synthesis via transcriptomics and constraint-based modeling.Bioprocess and biosystems engineering · 2026Article
- Efficient Expression of Lactone Hydrolase Cr2zen for Scalable Zearalenone Degradation inToxins · 2025Article
- Establishing a NovelBiomolecules · 2025Article
- The MFα signal sequence in yeast-based protein secretion: challenges and innovations'.Applied microbiology and biotechnology · 2025Review
- High-efficiency patatin expression strategies inSynthetic and systems biotechnology · 2025Article
- Structural Homology Fails to Predict Secretion Efficiency inInternational journal of molecular sciences · 2025Article
- Effect of Fold-Promoting Mutation and Signal Peptide Screening on Recombinant Glucan 1,4-Alpha-maltohydrolase Secretion in Pichia pastoris.Applied biochemistry and biotechnology · 2025Article
- Establishment and application of an RNAi system inFrontiers in bioengineering and biotechnology · 2025Article
- Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris.Applied microbiology and biotechnology · 2024Article
- Study of Potential Blocking Peptides Targeting the SARS-CoV-2 RBD/hACE2 Interaction.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Combined strategies for improving the heterologous expression of a novel xylanase fromSynthetic and systems biotechnology · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Pichia pastoris has been recognized as an important platform for the production of various heterologous proteins in recent years. The strong promoter AOX1, induced by methanol, with the help of the α-pre-pro signal sequence, can lead to a high expression level of extracellular protein. However, this combination was not always efficient, as protein secretion in P. pastoris involves numerous procedures mediated by several cellular proteins, including folding assisted by endoplasmic reticulum (ER) molecular chaperones, degradation through ubiquitination, and an efficient vesicular transport system. Efficient protein expression requires the cooperation of various intracellular pathways. This article summarizes the process of protein secretion, modification, and transportation in P. pastoris. In addition, the roles played by the key proteins in these processes and the corresponding co-expression effects are also listed. It is expected to lay the foundation for the industrial protein production of P. pastoris. KEY POINTS: • Mechanisms of chaperones in protein folding and their co-expression effects are summarized. • Protein glycosylation modifications are comprehensively reviewed. • Current dilemmas in the overall protein secretion pathway of Pichia pastoris and corresponding solutions are demonstrated.
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Registered trials
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