Evidence map›Paper›PMID 36038753›Full record

ArticleApplied microbiology and biotechnology2022

Life at the periphery: what makes CHO cells survival talents.

Tobias Jerabek, Florian Klingler, Nadja Raab, Nikolas Zeh, Jens Pfannstiel, Kerstin Otte

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Tobias Jerabek *Institute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach an der Riss, Germany. jerabek@hochschule-bc.de.ORCID http://orcid.org/0000-0001-9101-2565
Florian Klingler *Institute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach an der Riss, Germany.
Nadja RaabInstitute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach an der Riss, Germany.
Nikolas ZehInstitute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach an der Riss, Germany.
Jens PfannstielCore Facility Mass Spectrometry, University of Hohenheim, Stuttgart, Germany.
Kerstin OtteInstitute of Applied Biotechnology, University of Applied Sciences Biberach, Biberach an der Riss, Germany.
University of Applied Sciences Biberach · DEBoehringer Ingelheim (Germany) · DEUniversity of Hohenheim · DE

Funding

Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg 32-7545.20/4/25Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg IP2018/722674
6 · The paper itself

Abstract

The production of biopharmaceuticals relies on robust cell systems that can produce recombinant proteins at high levels and grow and survive in the stressful bioprocess environment. Chinese hamster ovary cells (CHO) as the main production hosts offer a variety of advantages including robust growth and survival in a bioprocess environment. Cell surface proteins are of special interest for the understanding of how CHO cells react to their environment while maintaining growth and survival phenotypes, since they enable cellular reactions to external stimuli and potentially initiate signaling pathways. To provide deeper insight into functions of this special cell surface sub-proteome, pathway enrichment analysis of the determined CHO surfaceome was conducted. Enrichment of growth/ survival-pathways such as the phosphoinositide-3-kinase (PI3K)-protein kinase B (AKT), mitogen-activated protein kinase (MAPK), Janus kinase/signal transducers and activators of transcription (JAK-STAT), and RAP1 pathways were observed, offering novel insights into how cell surface receptors and ligand-mediated signaling enable the cells to grow and survive in a bioprocess environment. When supplementing surfaceome data with RNA expression data, several growth/survival receptors were shown to be co-expressed with their respective ligands and thus suggesting self-induction mechanisms, while other receptors or ligands were not detectable. As data about the presence of surface receptors and their associated expressed ligands may serve as base for future studies, further pathway characterization will enable the implementation of optimization strategies to further enhance cellular growth and survival behavior. KEY POINTS: • PI3K/AKT, MAPK, JAK-STAT, and RAP1 pathway receptors are enriched on the CHO cell surface and downstream pathways present on mRNA level. • Detected pathways indicate strong CHO survival and growth phenotypes. • Potential self-induction of surface receptors and respective ligands.

Indexed as

Phosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktAnimalsCHO CellsCricetinaeCricetulusSignal TransductionPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktCell characterizationCell surface proteomeCHOGene expression analysisSurfaceomeSurvival pathway

Identifiers

PMID36038753
PMCPMC9468092
OpenAlexW4293679917

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.