Evidence map›Paper›PMID 36038685›Full record

ArticleDoklady. Biochemistry and biophysics2022

The Variable CTCF Site from Drosophila melanogaster Ubx Gene is Redundant and Has no Insulator Activity.

A N Ibragimov, O V Bylino, O V Kyrchanova, Y V Shidlovskii, R White, P Schedl, P G Georgiev

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Doklady. Biochemistry and biophysics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Molecular and genetic characterization ofmicroPublication biology · 2024
    Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

A N IbragimovInstitute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia. airat.ibra@gmail.com.
O V BylinoInstitute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
O V KyrchanovaInstitute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
Y V ShidlovskiiInstitute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
R WhiteUniversity of Cambridge, Cambridge, United Kingdom.
P SchedlPrinceton University, Princeton, USA.
P G GeorgievInstitute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
Institute of Gene Biology · RUPrinceton University · USUniversity of Cambridge · GB

Funding

Genetic regulatory mechanism in development and differentiationR35GM126975 · NIGMS · PRINCETON UNIVERSITY · PI SCHEDL, PAUL D · 2018 to 2022
$3.0M
NIGMS NIH HHS R35 GM126975
6 · The paper itself

Abstract

CTCF is the most thoroughly studied chromatin architectural protein and it is found in both Drosophila and mammals. CTCF preferentially binds to promoters and insulators and is thought to facilitate formation of chromatin loops. In a subset of sites, CTCF binding depends on the epigenetic status of the surrounding chromatin. One such variable CTCF site (vCTCF) was found in the intron of the Ubx gene, in close proximity to the BRE and abx enhancers. CTCF binds to the variable site in tissues where Ubx gene is active, suggesting that the vCTCF site plays a role in facilitating contacts between the Ubx promoter and its enhancers. Using CRISPR/Cas9 and attP/attB site-specific integration methods, we investigated the functional role of vCTCF and showed that it is not required for normal Drosophila development. Furthermore, a 2161-bp fragment containing vCTCF does not function as an effective insulator when substituted for the Fab-7 boundary in the Bithorax complex. Our results suggest that vCTCF function is redundant in the regulation of Ubx.

Indexed as

Drosophila melanogasterDrosophila ProteinsAnimalsCCCTC-Binding FactorChromatinDrosophilaHomeodomain ProteinsInsulator ElementsMammalsRepressor ProteinsTranscription FactorsCCCTC-Binding FactorChromatinCTCF protein, DrosophilaDrosophila ProteinsHomeodomain ProteinsRepressor ProteinsTranscription FactorsUbx protein, DrosophilaBithorax complexCTCFinsulatorsUbx

Identifiers

PMID36038685
PMCPMC9613721
OpenAlexW4293503369

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.