ArticleJournal of modern optics2022
Fluorescence and diffuse reflectance provide similar accuracy in recovering fluorophore concentration at short source-detector separations.
Article in Journal of modern optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Scaling method for efficient Monte Carlo simulation of fluorescence spectra from multi-layered turbid media with oblique illumination and collection geometry.Biomedical optics express · 2025Article
- First in human measurements of abscess cavity optical properties and methylene blue uptake prior to photodynamic therapy byJournal of biomedical optics · 2024Article
- Preliminary measurements of optical properties in human abscess cavities prior to methylene blue photodynamic therapy.Proceedings of SPIE--the International Society for Optical EngineeringArticle
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Abstract
Quantitative fluorescence spectroscopy requires corresponding reflectance measurements to correct for tissue absorption and scattering. However, it is unclear whether fluorescence adds value beyond the diffuse reflectance measurements necessary for correction. The goal of this study was to compare the accuracy of fluorescence and diffuse reflectance spectroscopy in recovering the concentration of a high-extinction fluorophore, methylene blue (MB), using a compact fiber-optic probe. Fluorescence and diffuse reflectance measurements of tissue simulating phantoms were made using a fiber-optic probe with source-detector separations of 288-1300 μm. Average error in recovered fluorophore concentration was 20.4% for fluorescence and 15.0% for reflectance, though this difference was not significant (p=0.77). Both methods returned concentrations that were similar to known MB concentrations (p≥0.79 in both cases). Fluorescence quantification of the concentration of a high extinction fluorophore did not significantly improve accuracy relative to diffuse reflectance. Investigators should consider whether fluorescence measurements are necessary for a given application.
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