Evidence map›Paper›PMID 36035791›Full record

ArticleSTAR protocols2022

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes.

Jacob Knittel, Gayathri Srinivasan, Carlye Frisch, Nicholas Brookhouser, Sreedevi Raman, Albert Essuman, David A Brafman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jacob KnittelSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
Gayathri SrinivasanSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
Carlye FrischSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
Nicholas BrookhouserSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA; Graduate Program in Clinical Translational Sciences, University of Arizona College of Medicine-Phoenix, Phoenix, AZ 85004, USA.
Sreedevi RamanSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
Albert EssumanSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.
David A BrafmanSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA. Electronic address: david.brafman@asu.edu.

Funding

A Pluripotent Stem Cell-Based Model to Investigate the Mechanisms of TBI-Induced ADR21AG063358 · NIA · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI BRAFMAN, DAVID A, STABENFELDT, SARAH E · 2019 to 2020
$434k
Using hiPSCs to investigate the protective mechanisms of the ApoEch mutationR21AG070406 · NIA · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI BRAFMAN, DAVID A · 2021 to 2021
$432k
Elucidating the protective effects of the KL-VS variant using isogenic hiPSCsR21AG075612 · NIA · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI BRAFMAN, DAVID A · 2022 to 2022
$432k
Generation and characterization of isogenic hiPSC lines with various APOE genotypesR21AG056706 · NIA · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI BRAFMAN, DAVID A · 2017 to 2018
$409k
NIA NIH HHS R21 AG056706NIA NIH HHS R21 AG063358NIA NIH HHS R21 AG070406NIA NIH HHS R21 AG075612
6 · The paper itself

Abstract

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

Indexed as

Induced Pluripotent Stem CellsAstrocytesCell DifferentiationCells, CulturedHumansNeuronsCell differentiationNeuroscienceStem cellsTissue engineering

Identifiers

PMID36035791
PMCPMC9405537

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.