Evidence map›Paper›PMID 36035789›Full record

ArticleSTAR protocols2022

Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue.

Kenta Yamauchi, Takahiro Furuta, Shinichiro Okamoto, Megumu Takahashi, Masato Koike, Hiroyuki Hioki

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Kenta YamauchiDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan.
Takahiro FurutaDepartment of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
Shinichiro OkamotoDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, Japan.
Megumu TakahashiDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Neuroscience, Graduate School of Medicine, Kyoto University, Kyoto, Tokyo 606-8501, Japan; Japan Society for the Promotion of Science (JSPS), Chiyoda-ku, Tokyo 102-0083, Japan.
Masato KoikeDepartment of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Advanced Research Institute for Health Sciences, Juntendo University, Bunkyo-Ku, Tokyo 113-8421, Japan.
Hiroyuki HiokiDepartment of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan. Electronic address: h-hioki@juntendo.ac.jp.
Juntendo University · JPJapan Society for the Promotion of Science · JPOsaka University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale light microscopy (LM) with electron microscopy (EM) in mouse brain tissue. We describe tissue slice preparation and LM/EM dual labeling with EGFP-APEX2 fusion protein. We then detail Sca

Indexed as

BrainNeuronsAnimalsMiceMicroscopy, ElectronCell BiologyMicroscopyNeuroscience

Identifiers

PMID36035789
PMCPMC9405099
OpenAlexW4293717806

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.