Evidence map›Paper›PMID 36035788›Full record

ArticleSTAR protocols2022

Transcriptional profiling of mouse projection neurons with VECTORseq.

Victoria Cheung, Philip Chung, Evan H Feinberg

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Victoria CheungDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA; Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
Philip ChungDepartment of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA 98195, USA.
Evan H FeinbergDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA; Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: evan.feinberg@ucsf.edu.
University of California, San Francisco · USUniversity of Washington · US

Funding

Virology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Monica Gandhi · 1988 to 2026
$93.6M
High-resolution neural circuit dissection with controllers locally affecting synaptic partners (CLASP)DP2MH119426 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FEINBERG, EVAN HARRIMAN · 2018 to 2018
$2.4M
Defining cell types that control action selection and executionR01NS109060 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FEINBERG, EVAN HARRIMAN · 2019 to 2023
$1.9M
BD FACSAria II Flow CytometerS10RR028962 · NCRR · J. DAVID GLADSTONE INSTITUTES · PI CAVROIS, MARIELLE · 2011 to 2011
$544k
NCRR NIH HHS S10 RR028962NIAID NIH HHS P30 AI027763NIMH NIH HHS DP2 MH119426NINDS NIH HHS R01 NS109060
6 · The paper itself

Abstract

Existing techniques for transcriptional profiling of projection neurons could be applied to only one neuronal population per experiment. To increase throughput, we developed VECTORseq, which repurposes retrogradely infecting viruses to deliver multiplexable RNA barcodes, enabling projection anatomy to be read out in single-cell datasets. In this protocol, we describe the delivery of viral barcodes to mouse brain to label different projection neurons. We then detail single-cell or nuclei isolation for sequencing, followed by the analysis of single-cell sequencing data. For complete details on the use and execution of this protocol, please refer to Cheung et al. (2021).

Indexed as

High-Throughput Nucleotide SequencingRNAAnimalsInterneuronsMiceNeuronsSequence Analysis, RNARNABioinformaticsCell isolationGene expressionGenomicsNeuroscienceRNAseqSequence analysisSequencingSingle cell

Identifiers

PMID36035788
PMCPMC9405111
OpenAlexW4293772125

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.