Evidence map›Paper›PMID 36033826›Full record

ArticleDisease markers2022

Clinical Value Screening, Prognostic Significance, and Key Gene Identification of TrkB in Laryngeal Carcinoma.

Jing Zhang, Xiaoyan Hu, Shuang Liu, Yuxiao He, Liangge Lyu, Liang Jiang

Abstract read
PubMed Full text at PMC Publisher
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Article in Disease markers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jing ZhangDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.ORCID https://orcid.org/0000-0002-4258-2278
Xiaoyan HuDepartment of Pathogen Biology, School of Basic Medicine Southwest Medical University, China.ORCID https://orcid.org/0000-0003-1241-5695
Shuang LiuDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Yuxiao HeDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Liangge LyuDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Liang JiangDepartment of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.ORCID https://orcid.org/0000-0001-6448-4479

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Using human gene chip expression profiling technology to screen out downstream genes related to TrkB regulation in laryngeal cancer cells. Methods: Using the Hep-2 TrkB shRNA cell line, divide it into an experimental group (shNTRK2) and a control group (PLKO1), and use the human gene expression microarray to screen out the differential genes. Then, select 10 upregulated genes and 10 downregulated genes from the differential genes, and use RT-PCR to verify whether the screening results of human gene expression microarray profiles are reliable. Use GO, KEGG, and miRNA enrichment analyses, PPI network diagram, etc., to analyze the differential genes and further screen out the key genes. Results: A total of 318 differential genes (87 upregulated genes and 231 downregulated genes) were screened in laryngeal cancer cells. Use RT-PCR for the 10 upregulated differential genes (DMKN, FHL1, FOXN4, GGNBP1, HOXB9, ABCB1, TNFAI, RGS2, LINC01133, and FGG) and 10 downregulated differential genes (CHI3L1, FMOD, IGFBP1, IRF5, SPARC, NPAS4, TRPS1, TRAP, COL8A1, and DNER), and the results are consistent with the chip results, confirming the accuracy of the chip results; GO analysis results show that the downstream differential genes (DEGs) regulated by TrkB are mainly involved in biological processes such as retinol metabolic process, diterpenoid metabolic process, and regulation of cell-substrate adhesion. DEGs mainly affect cytoskeletal protein binding, serotonin-activated cation-selective channel activity, and sphingosine molecular functions. DEGs are mainly enriched in the cell periphery, secretory granule, cytoplasmic membrane-bounded vesicle lumen, blood microparticle, and other molecular components. The results of disease enrichment analysis show that the downstream differential genes regulated by TrkB are mainly involved in atypical hemolytic uremic syndrome, hematologic disease, meningococcal disease, lung cancer, susceptibility, asthma, and other diseases. The PPI network diagram results showed 7 hub genes, and then, we used GO analysis and KEGG enrichment analysis to see the biological process, cell component, molecular functions, and biological pathways. Conclusion: Gene chip technology was used to screen out the differential genes of TrkB epigenetic modification in the Hep-2 cell line, and seven key genes (ALDH1A1, SDR16C5, PIK3R1, PLCG2, IL2RG, PIK3CD, and SPARC) were further screened using bioinformatics technology.

Indexed as

CarcinomaLaryngeal NeoplasmsGene Expression ProfilingGene Expression Regulation, NeoplasticGene Regulatory NetworksHomeodomain ProteinsHumansIntracellular Signaling Peptides and ProteinsLIM Domain ProteinsMuscle ProteinsPrognosisRepressor ProteinsFHL1 protein, humanHomeodomain ProteinsHOXB9 protein, humanIntracellular Signaling Peptides and ProteinsLIM Domain ProteinsMuscle ProteinsRepressor ProteinsTRPS1 protein, human

Identifiers

PMID36033826
PMCPMC9417763

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.