ArticleSensors and actuators. B, Chemical2022
Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection.
Article in Sensors and actuators. B, Chemical, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 101 citations in OpenAlex.
- Transport-Reaction-Signal Coupling in Lateral Flow Assays for Next-Generation Point-of-Care Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Towards deployable CRISPR-based nucleic acid detection.Progress in biomedical engineering (Bristol, England) · 2026Review
- Application of CRISPR-Cas12a/Cas13a dual enzyme cleavage technology in the differential diagnosis of Chikungunya virus and Dengue virus.Journal of biological engineering · 2026Article
- Application progress and biosafety challenges of gene editing and synthetic biotechnology in diagnosis, treatment and prevention of infectious diseases.Biosafety and health · 2025Review
- Advancements and Applications of Lateral Flow Assays (LFAs): A Comprehensive Review.Sensors (Basel, Switzerland) · 2025Review
- CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation.Military Medical Research · 2025Article
- Orthogonal CRISPR/Cas system facilitated dual-color fluorescence fiber-embedded optofluidic nano-biochip for parallel amplification-free on-site detection of bacterium and virus.Mikrochimica acta · 2025Article
- Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops.Journal of advanced research · 2025Article
- On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay.iScience · 2024Article
- Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay.BMC veterinary research · 2024Article
- SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control.Sensors & diagnostics · 2024Article
- Ov-RPA-CRISPR/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces.Parasites & vectors · 2024Article
- A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis.Parasites & vectors · 2024Article
- Rapid detection ofFrontiers in microbiology · 2024Article
- Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis.Frontiers in cellular and infection microbiology · 2024Review
- Dual-clamped one-pot SERS-based biosensors for rapid and sensitive detection of SARS-CoV-2 using portable Raman spectrometer.Sensors and actuators. B, Chemical · 2023Article
- RNA extraction-free workflow integrated with a single-tube CRISPR-Cas-based colorimetric assay for rapid SARS-CoV-2 detection in different environmental matrices.Journal of hazardous materials · 2023Article
- Rapid detection of grass carp reovirus type 1 using RPA-based test strips combined with CRISPR Cas13a system.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of field-deployable detection platform amenable for multiplexed genes testing will significantly improve the efficiency and reliability during point-of-care testing (POCT) applications. In this regard, an orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (designated as OC-MLFA) is proposed for SARS-CoV-2 genome detection. Taking the advantage of activation and cleavage preferences between Cas12a and Cas13a, orthogonal (two-independent-channel signal readout) CRISPR-Cas system is investigated. Lateral flow strips with two target lines are designed to accommodate the orthogonal CRISPR system. The interference between Cas12a and Cas13a channels can be effectively eliminated via the elaborate nucleic acids and lateral flow strips design. The high preamplification efficiency from reverse transcription recombinase polymerase amplification (RT-RPA) and Cas enzyme mediated
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.