Evidence map›Paper›PMID 36031653›Full record

ArticleMikrochimica acta2022

Electrochemical aptasensor for Staphylococcus aureus by stepwise signal amplification.

Huiqian Zhou, Wenbo Guo, Shian Wang, Tingting Hao, Zhaoliang Wang, Yufang Hu, Sui Wang, Jianjun Xie, Xiaohua Jiang, Zhiyong Guo

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Huiqian ZhouState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.
Wenbo GuoState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.
Shian WangFaculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, 315211, People's Republic of China.
Tingting HaoState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.
Zhaoliang WangFaculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, 315211, People's Republic of China.
Yufang HuState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.
Sui WangState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.
Jianjun XieFaculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, 315211, People's Republic of China. xiejianjun@nbu.edu.cn.
Xiaohua JiangSchool of Materials & Environmental Engineering, Shenzhen Polytechnic, Shenzhen, 518055, People's Republic of China. jiangxiaohua@szpt.edu.cn.
Zhiyong GuoState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, People's Republic of China. guozhiyong@nbu.edu.cn.ORCID http://orcid.org/0000-0003-1288-1573
Ningbo University · CNShenzhen Polytechnic University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An electrochemical aptasensor for ultrasensitive detection of Staphylococcus aureus (SA) has been developed based on stepwise signal amplification. In the sample processing stage, the specific recognition between SA and aptamer triggers the enzyme-assisted cyclic cleavage to produce a large amount of target DNA (tDNA), realizing the first-level signal amplification. In the sensor assembly stage, tDNA induces a catalytic hairpin assembly (CHA) cycle to capture much more hairpin DNA H2 labeled by the electrochemical tag ferrocene, bringing the second-level signal amplification. In the signal detection stage, ferrocene is quasi-adsorbed on the electrode surface, and a high redox peak current linearly increasing with the scan rate up to 1000 V/s has been obtained by fast scan cyclic voltammetry (FSCV), achieving the third-level signal amplification. Under the optimized experimental conditions, the linear range and detection limit are 1 ~ 10

Indexed as

Aptamers, NucleotideBiosensing TechniquesDNAElectrochemical TechniquesGoldMetallocenesReproducibility of ResultsStaphylococcus aureusAptamers, NucleotideDNAGoldMetallocenesCatalytic hairpin assemblyElectrochemical aptasensorEnzyme-assisted cyclic cleavageFast scan cyclic voltammetryStaphylococcus aureusStepwise signal amplification

Identifiers

PMID36031653
OpenAlexW4293413271

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.