ArticleG3 (Bethesda, Md.)2022
The mIAA7 degron improves auxin-mediated degradation in Caenorhabditiselegans.
Article in G3 (Bethesda, Md.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Regulatory architecture controlling terminal differentiation of an interoceptive paraneuron in Caenorhabditis elegans.Development (Cambridge, England) · 2026Article
- Regulatory architecture controlling terminal differentiation of an interoceptive paraneuron inbioRxiv : the preprint server for biology · 2026Article
- The Caenorhabditis Genetics Center Curated Special Collections: a guide to protein degradation systems.Genetics · 2026Review
- A conserved C. elegans zinc finger homeodomain protein, ZFH-2, is continuously required for the structural integrity and function of the alimentary tract and gonad.Development (Cambridge, England) · 2026Article
- Dietary restriction shapes intergenerational ribosome abundance and early growth of Caenorhabditis elegans offspring.PLoS biology · 2026Article
- A conservedbioRxiv : the preprint server for biology · 2026Article
- Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation System.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Engineering the auxin-inducible degron system for tunable in vivo control of organismal physiology.Nature communications · 2025Article
- The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential.Development (Cambridge, England) · 2025Article
- SunTag-Based Single-Molecule Translation Imaging inBio-protocol · 2025Article
- Nuclear lamina-associated domain biogenesis is regulated by nuclear pore density during embryogenesis and mediates UV protection.bioRxiv : the preprint server for biology · 2025Article
- Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast.Journal of visualized experiments : JoVE · 2025Article
- Caenorhabditis elegans LET-381 and DMD-4 control development of the mesodermal HMC endothelial cell.Development (Cambridge, England) · 2025Article
- An auxin-inducible degron system for conditional mutation in the fungal meningitis pathogen Cryptococcus neoformans.G3 (Bethesda, Md.) · 2025Article
- Measuring and manipulating localized translation of erm-1 in the C. elegans embryo.Development (Cambridge, England) · 2025Article
- Protein-targeting reverse genetic approaches: the future of oocyte and preimplantation embryo research.Molecular human reproduction · 2025Review
- Review
- ZIF-1-mediated degradation of zinc finger proteins in the Caenorhabditis elegans germ line.Genetics · 2023Article
- ZIF-1-mediated degradation of endogenous and heterologous zinc finger proteins in thebioRxiv : the preprint server for biology · 2023Article
- An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in Caenorhabditis elegans.Genetics · 2023Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Auxin-inducible degradation is a powerful tool for the targeted degradation of proteins with spatiotemporal control. One limitation of the auxin-inducible degradation system is that not all proteins are degraded efficiently. Here, we demonstrate that an alternative degron sequence, termed mIAA7, improves the efficiency of degradation in Caenorhabditiselegans, as previously reported in human cells. We tested the depletion of a series of proteins with various subcellular localizations in different tissue types and found that the use of the mIAA7 degron resulted in faster depletion kinetics for 5 out of 6 proteins tested. The exception was the nuclear protein HIS-72, which was depleted with similar efficiency as with the conventional AID* degron sequence. The mIAA7 degron also increased the leaky degradation for 2 of the tested proteins. To overcome this problem, we combined the mIAA7 degron with the C. elegans AID2 system, which resulted in complete protein depletion without detectable leaky degradation. Finally, we show that the degradation of ERM-1, a highly stable protein that is challenging to deplete, could be improved further by using multiple mIAA7 degrons. Taken together, the mIAA7 degron further increases the power and applicability of the auxin-inducible degradation system. To facilitate the generation of mIAA7-tagged proteins using CRISPR/Cas9 genome engineering, we generated a toolkit of plasmids for the generation of dsDNA repair templates by PCR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.