ArticleNature communications2022
Water-organizing motif continuity is critical for potent ice nucleation protein activity.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Ice nucleation by kaolinite: Morphology shapes the size dependence of ice nucleation active sites on dust particles.Science advances · 2026Article
- Design Principles for β-Solenoid Stability via Covalent and Electrostatic Capping Motifs.The journal of physical chemistry letters · 2026Article
- Biochemical characterization of a fungal ice nucleation protein from Podila clonocystis.Scientific reports · 2026Article
- A previously unrecognized class of fungal ice-nucleating proteins with bacterial ancestry.Science advances · 2026Article
- Article
- How to measure the activity of antifreeze proteins?Biophysical reviews · 2025Review
- A Novel Gene Synthesis Platform for Designing Functional Protein Polymers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges.Biomicrofluidics · 2025Review
- Hierarchical assembly and environmental enhancement of bacterial ice nucleators.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recent Advances in Antifreeze Peptide Preparation: A Review.Molecules (Basel, Switzerland) · 2024Review
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.eLife · 2023Article
- Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C.bioRxiv : the preprint server for biology · 2023Article
- Review
- Poly(vinyl alcohol) Molecular Bottlebrushes Nucleate Ice.Biomacromolecules · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Bacterial ice nucleation proteins (INPs) can cause frost damage to plants by nucleating ice formation at high sub-zero temperatures. Modeling of Pseudomonas borealis INP by AlphaFold suggests that the central domain of 65 tandem sixteen-residue repeats forms a beta-solenoid with arrays of outward-pointing threonines and tyrosines, which may organize water molecules into an ice-like pattern. Here we report that mutating some of these residues in a central segment of P. borealis INP, expressed in Escherichia coli, decreases ice nucleation activity more than the section's deletion. Insertion of a bulky domain has the same effect, indicating that the continuity of the water-organizing repeats is critical for optimal activity. The ~10 C-terminal coils differ from the other 55 coils in being more basic and lacking water-organizing motifs; deletion of this region eliminates INP activity. We show through sequence modifications how arrays of conserved motifs form the large ice-nucleating surface required for potency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.