ArticlePloS one2022
Host induced gene silencing of the Sclerotinia sclerotiorum ABHYDROLASE-3 gene reduces disease severity in Brassica napus.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Nano-enabled spray-induced RNA interference against fungal pathogens of oilseed crops: carrier performance, delivery evidence and field translation.World journal of microbiology & biotechnology · 2026Review
- Dual RNA sequencing reveals the transcriptomic and cellular response of Cannabis sativa to infection by the fungal pathogen Sclerotinia sclerotiorum.Scientific reports · 2026Article
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- Integrated Pest Management of Sclerotinia Stem Rot in Soybean: Current Strategies and Future Prospects.Journal of fungi (Basel, Switzerland) · 2025Review
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The SsSmk1-SsSom1-SsMsb2 Pathway Regulates Infection Cushion Formation and Pathogenicity in Sclerotinia sclerotiorum.Molecular plant pathology · 2025Article
- A GDP-mannose-1-phosphate guanylyltransferase as a potential HIGS target against Sclerotinia sclerotiorum.PLoS pathogens · 2025Article
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- Global mRNA profiling reveals the effect of boron as a crop protection tool againstAoB PLANTS · 2024Article
- RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry.Plant cell reports · 2024Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Genome sequencing and comparative genomics reveal insights into pathogenicity and evolution of Fusarium zanthoxyli, the causal agent of stem canker in prickly ash.BMC genomics · 2024Article
- Control of Sclerotinia sclerotiorum via an RNA interference (RNAi)-mediated targeting of SsPac1 and SsSmk1.Planta · 2024Article
- RAS signalling genes can be used as host-induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea.Plant biotechnology journal · 2024Article
- Mining theJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Control of white mold (Sclerotinia sclerotiorum) through plant-mediated RNA interference.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sclerotinia sclerotiorum is a pathogenic fungus that infects hundreds of crop species, causing extensive yield loss every year. Chemical fungicides are used to control this phytopathogen, but with concerns about increasing resistance and impacts on non-target species, there is a need to develop alternative control measures. In the present study, we engineered Brassica napus to constitutively express a hairpin (hp)RNA molecule to silence ABHYRDOLASE-3 in S. sclerotiorum. We demonstrate the potential for Host Induced Gene Silencing (HIGS) to protect B. napus from S. sclerotiorum using leaf, stem and whole plant infection assays. The interaction between the transgenic host plant and invading pathogen was further characterized at the molecular level using dual-RNA sequencing and at the anatomical level through microscopy to understand the processes and possible mechanisms leading to increased tolerance to this damaging necrotroph. We observed significant shifts in the expression of genes relating to plant defense as well as cellular differences in the form of structural barriers around the site of infection in the HIGS-protected plants. Our results provide proof-of-concept that HIGS is an effective means of limiting damage caused by S. sclerotiorum to the plant and demonstrates the utility of this biotechnology in the development of resistance against fungal pathogens.
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Registered trials
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