ArticleJournal of the American Society for Mass Spectrometry2022
Attribute Analytics Performance Metrics from the MAM Consortium Interlaboratory Study.
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Article in Journal of the American Society for Mass Spectrometry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Development and Integrated Application of the Multi-Attribute Method (MAM) in Quality Control of Biotechnological Drugs.Biotechnology and bioengineering · 2026Review
- Protein Therapeutic Quality Control Using Multi-Attribute Method (MAM): Challenges and Current Practice in cGMP Environments.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Polarity- and Sequence-Dependent Ionization of Therapeutic Antibody-siRNA Conjugates: Enabling Intact Multi-attribute Method for Comprehensive Characterization and Identity Release Assay.Analytical chemistry · 2026Article
- Article
- Functionality and translation fidelity characterization of mRNA vaccines using platform based mass spectrometry detection.NPJ vaccines · 2025Article
- The Accurate Prediction of Antibody Deamidations by Combining High-Throughput Automated Peptide Mapping and Protein Language Model-Based Deep Learning.Antibodies (Basel, Switzerland) · 2024Article
- Article
Corrections and comments
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Authors and funding
97 authors at 20 institutions in 4 countries.
Trina MouchahoirNational Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, United States.ORCID 0000-0002-6790-830X
John E SchielNational Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, United States.
Rich RogersJust-Evotech Biologics, Inc., 401 Terry Ave N., Seattle, Washington 98109, United States.
Alan HeckertNational Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, United States.ORCID 0000-0002-8430-6757
Benjamin J PlaceNational Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, United States.ORCID 0000-0003-0953-5215
Aaron AmmermanAbbVie, 1000 Gateway Blvd, South San Francisco, California 94080, United States.
Xiaoxiao LiAbbVie, 1000 Gateway Blvd, South San Francisco, California 94080, United States.
Tom RobinsonAbbVie, 1000 Gateway Blvd, South San Francisco, California 94080, United States.
Brian SchmidtAbbVie, 1000 Gateway Blvd, South San Francisco, California 94080, United States.
Chris M ChumsaeAbbVie, 100 Research Drive, Worcester, Massachusetts 01605, United States.
Xinbi LiAbbVie, 100 Research Drive, Worcester, Massachusetts 01605, United States.
Anton V ManuilovAbbVie, 100 Research Drive, Worcester, Massachusetts 01605, United States.
Bo YanAbbVie, 100 Research Drive, Worcester, Massachusetts 01605, United States.
Gregory O StaplesAgilent Technologies, 5301 Stevens Creek Blvd, Santa Clara, California 95008, United States.
Da RenAmgen, One Amgen Center Dr, Thousand Oaks, California 91320, United States.
Alexander J VeachAmgen, One Amgen Center Dr, Thousand Oaks, California 91320, United States.
Dongdong WangBioAnalytix, 790 Memorial Dr, Cambridge, Massachusetts 02139, United States.
Wael YaredBioAnalytix, 790 Memorial Dr, Cambridge, Massachusetts 02139, United States.
Zoran SosicBiogen, 125 Broadway, Cambridge, Massachusetts 02142, United States.
Yan WangBiogen, 125 Broadway, Cambridge, Massachusetts 02142, United States.
Li ZangBiogen, 125 Broadway, Cambridge, Massachusetts 02142, United States.
Anthony M LeoneBristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.
Peiran LiuBristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.
Richard LudwigBristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.
Li TaoBristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.ORCID 0000-0002-9343-7011
Wei WuBristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, New Jersey 08534, United States.
Ahmet CansizogluCharles River Laboratories, 8 Henshaw Street, Shrewsbury, Massachusetts 01801, United States.
Andrew HannemanCharles River Laboratories, 8 Henshaw Street, Shrewsbury, Massachusetts 01801, United States.
Greg W AdamsFUJIFILM Diosynth Biotechnologies, 101 J. Morris Commons Ln, Morrisville, North Carolina 27560, United States.
Irina PerdivaraFUJIFILM Diosynth Biotechnologies, 101 J. Morris Commons Ln, Morrisville, North Carolina 27560, United States.
Hunter WalkerFUJIFILM Diosynth Biotechnologies, 101 J. Morris Commons Ln, Morrisville, North Carolina 27560, United States.
Margo WilsonFUJIFILM Diosynth Biotechnologies, 101 J. Morris Commons Ln, Morrisville, North Carolina 27560, United States.
Arnd BrandenburgGenedata, Margarethenstrasse 38, Basel, 4053, Switzerland.
Nick DeGraan-WeberGenedata, 750 Marrett Road, One Cranberry Hill, Lexington, Massachusetts 02421, United States.
Stefano GottaGenedata, Margarethenstrasse 38, Basel, 4053, Switzerland.
Joe ShambaughGenedata, 750 Marrett Road, One Cranberry Hill, Lexington, Massachusetts 02421, United States.
Melissa AlvarezGenentech, 1 DNA Way, South San Francisco, California 94080, United States.
X Christopher YuGenentech, 1 DNA Way, South San Francisco, California 94080, United States.
Li CaoGSK, 709 Swedeland Rd, King of Prussia, Pennsylvania 19406, United States.
Chun ShaoGSK, 709 Swedeland Rd, King of Prussia, Pennsylvania 19406, United States.
Andrew MahanJanssen, 1400 McKean Road, Springhouse, Pennsylvania 19477, United States.
Hirsh NandaJanssen, 1400 McKean Road, Springhouse, Pennsylvania 19477, United States.
Kristen NieldsJanssen, 1400 McKean Road, Springhouse, Pennsylvania 19477, United States.
Nancy NightlingerJust-Evotech Biologics, Inc., 401 Terry Ave N., Seattle, Washington 98109, United States.
Ben NiuAstraZeneca, One MedImmune Way, Gaithersburg, Maryland 20878, United States.
Jihong WangAstraZeneca, One MedImmune Way, Gaithersburg, Maryland 20878, United States.
Wei XuAstraZeneca, One MedImmune Way, Gaithersburg, Maryland 20878, United States.
Gabriella LeoEMD Serono an affiliate of Merck KGaA, Darmstadt, Germany, Via Luigi Einaudi 11, Guidonia Montecelio (Roma), 00012, Italy.
Nunzio SepeEMD Serono an affiliate of Merck KGaA, Darmstadt, Germany, Via Luigi Einaudi 11, Guidonia Montecelio (Roma), 00012, Italy.
Yan-Hui LiuMerck & Co., Inc.., 2000 Galloping Hill Rd, Kenilworth, New Jersey 07033, United States.
Bhumit A PatelMerck & Co., Inc.., 2000 Galloping Hill Rd, Kenilworth, New Jersey 07033, United States.ORCID 0000-0002-7970-7759
Douglas RichardsonMerck & Co., Inc.., 2000 Galloping Hill Rd, Kenilworth, New Jersey 07033, United States.ORCID 0000-0001-8488-6758
Yi WangMerck & Co., Inc.., 2000 Galloping Hill Rd, Kenilworth, New Jersey 07033, United States.
Daniela TizabiNational Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, Maryland 20899, United States.
Oleg V BorisovNovavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878, United States.
Yali LuNovavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878, United States.
Ernest L MaynardNovavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878, United States.
Albrecht GruhlerNovo Nordisk, Novo Nordisk Park, Måløv 2760, Denmark.
Kim F HaselmannNovo Nordisk, Novo Nordisk Park, Måløv 2760, Denmark.ORCID 0000-0001-8031-3979
Thomas N KroghNovo Nordisk, Novo Nordisk Park, Måløv 2760, Denmark.
Carsten P SönksenNovo Nordisk, Novo Nordisk Park, Måløv 2760, Denmark.
Simon LetartePfizer, 375 N Field Dr, Lake Forest, Illinois 60045, United States.
Sean ShenPfizer, 375 N Field Dr, Lake Forest, Illinois 60045, United States.
Kristin BoggioPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
Keith JohnsonPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
Wenqin NiPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
Himakshi PatelPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
David RipleyPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
Jason C RousePfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.ORCID 0000-0002-2721-7264
Ying ZhangPfizer, 1 Burtt Rd, Andover, Massachusetts 01810, United States.
Carly DanielsPfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Andrew DawdyPfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Olga FriesePfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Thomas W PowersPfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Justin B SperryPfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Josh WoodsPfizer, 700 Chesterfield Pkwy West, Chesterfield, Missouri 63017, United States.
Eric CarlsonProtein Metrics, Inc., 20863 Stevens Creek Blvd, Cupertino, California 95014, United States.
K Ilker SenProtein Metrics, Inc., 20863 Stevens Creek Blvd, Cupertino, California 95014, United States.ORCID 0000-0003-0756-9518
St John SkiltonProtein Metrics, Inc., 20863 Stevens Creek Blvd, Cupertino, California 95014, United States.
Michelle BuschSanofi, 1 The Mountain Rd, Framingham, Massachusetts 01701, United States.ORCID 0000-0003-4867-6280
Anders LundSanofi, 1 The Mountain Rd, Framingham, Massachusetts 01701, United States.
Martha StapelsSanofi, 1 The Mountain Rd, Framingham, Massachusetts 01701, United States.
Xu GuoSCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, Canada.
Sibylle HeidelbergerSCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, Canada.
Harini KaluarachchiSCIEX, 71 Four Valley Drive, Concord, ON L4K 4V8, Canada.
Sean McCarthySCIEX, 500 Old Connecticut Path, Framingham, Massachusetts 01701, United States.
John KimTeva, 145 Brandywine Pkwy, West Chester, Pennsylvania 19380, United States.
Jing ZhenTeva, 145 Brandywine Pkwy, West Chester, Pennsylvania 19380, United States.
Ying ZhouTeva, 145 Brandywine Pkwy, West Chester, Pennsylvania 19380, United States.
Sarah RogstadU.S. Food and Drug Administration, 10903 New Hampshire Ave, Silver Spring, Maryland 20993, United States.ORCID 0000-0002-8998-053X
Xiaoshi WangU.S. Food and Drug Administration, 10903 New Hampshire Ave, Silver Spring, Maryland 20993, United States.
Jing FangWaters, 34 Maple St, Milford, Massachusetts 01757, United States.
Weibin ChenWaters, 34 Maple St, Milford, Massachusetts 01757, United States.
Ying Qing YuWaters, 34 Maple St, Milford, Massachusetts 01757, United States.
John G HoogerheideZoetis, 333 Portage St, Kalamazoo, Michigan 49007, United States.
Rebecca ScottZoetis, 333 Portage St, Kalamazoo, Michigan 49007, United States.
Hua YuanZoetis, 333 Portage St, Kalamazoo, Michigan 49007, United States.
Pfizer (United States) · USAbbVie (United States) · USBristol-Myers Squibb (United States) · USMerck & Co., Inc., Rahway, NJ, USA (United States) · USNational Institute of Standards and Technology · USNovo Nordisk (Denmark) · DKAstraZeneca (United States) · USNovavax (United States) · USProtein Metrics (United States) · USSanofi (United States) · USSpringhouse · USTeva Pharmaceuticals (United States) · USWaters (United States) · USZoetis (United States) · USAmgen (United States) · USBiogen (United States) · USCharles River Laboratories (United States) · USEvotec (United States) · USGenedata (Switzerland) · CHMerck (Germany) · DE
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multi-attribute method (MAM) was conceived as a single assay to potentially replace multiple single-attribute assays that have long been used in process development and quality control (QC) for protein therapeutics. MAM is rooted in traditional peptide mapping methods; it leverages mass spectrometry (MS) detection for confident identification and quantitation of many types of protein attributes that may be targeted for monitoring. While MAM has been widely explored across the industry, it has yet to gain a strong foothold within QC laboratories as a replacement method for established orthogonal platforms. Members of the MAM consortium recently undertook an interlaboratory study to evaluate the industry-wide status of MAM. Here we present the results of this study as they pertain to the targeted attribute analytics component of MAM, including investigation into the sources of variability between laboratories and comparison of MAM data to orthogonal methods. These results are made available with an eye toward aiding the community in further optimizing the method to enable its more frequent use in the QC environment.
Indexed as
ProteinsMass SpectrometryPeptide MappingQuality ControlProteinsattribute analyticsMAM Consortiummulti-attribute methodNISTmAbtargeted analytics
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