ArticleFrontiers in bioengineering and biotechnology2022
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
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- Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.Molecular microbiology · 2026Article
- Tissue-specific recombination-activated clonal and inducible transcription (TRAC-IT) enables controlled gene activation in stably marked tissues.The Plant journal : for cell and molecular biology · 2026Article
- Quantifying the Acute Toxicity of Alpha-Synuclein Membrane-Accumulation in a Cell-Based Assay.Journal of molecular neuroscience : MN · 2026Article
- TheJournal of proteome research · 2026Article
- Quantifying the Acute Toxicity of Alpha-Synuclein Membrane-Accumulation in a Cell-Based Assay.Research square · 2026Article
- Boosting Gene Translation by a Short ORF Encoding for a "Nonsense" Peptide Positioned Immediately Upstream.ACS synthetic biology · 2025Article
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Genetically encoded fluorescent reporter for polyamines.Nature communications · 2025Article
- Codon usage modulates the relationship between the burden and yield of protein overexpression.Journal of biological engineering · 2025Article
- Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs.Frontiers in bioengineering and biotechnology · 2025Article
- Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins.BMC plant biology · 2024Article
- Image-guided optogenetic spatiotemporal tissue patterning using μPatternScope.Nature communications · 2024Article
- Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.Nucleic acids research · 2024Article
- Article
- Standard Intein Gene Expression Ramps (SIGER) for Protein-Independent Expression Control.ACS synthetic biology · 2023Article
- Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2Scientific reports · 2022Article
- Tunable transcription factor library for robust quantification of regulatory properties in Escherichia coli.Molecular systems biology · 2022Article
- Identification and Cross-Characterisation of Artificial Promoters and 5' Untranslated Regions inFrontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform in both prokaryotes and eukaryotes. The short isoform creates significant background fluorescence that biases the outcome of expression studies. In this study, we identified the short protein isoform, traced its origin, and determined the extent of the issue within the family of red fluorescent protein. Our analysis showed that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. We provided a re-engineered
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.