ArticleLife (Basel, Switzerland)2022
Cryopreservation of Pig Semen Using a Quercetin-Supplemented Freezing Extender.
Article in Life (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Methyl Gallate Enhances Post-Thaw Boar Sperm Quality by Alleviating Oxidative Stress and Preserving Mitochondrial Function.Antioxidants (Basel, Switzerland) · 2025Article
- Enhancing Stallion Semen Cryopreservation: Selected Antioxidant Extracts and Sperm Freezability.Antioxidants (Basel, Switzerland) · 2025Article
- Quercetin-coated CuO-ZnO nanoparticles enhance post-thaw quality of ram semen.Scientific reports · 2025Article
- Article
- Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights.Animals : an open access journal from MDPI · 2025Review
- Comparative Analysis of Classic Semen Extenders for Frozen-Thawed Boar Semen.Animals : an open access journal from MDPI · 2025Article
- Effect of idebenone supplementation in a semen extender on boar spermatozoa quality during liquid storage.Veterinary world · 2025Article
- Effect of Centrifuged Chicken Egg Yolk on the Cryopreservation of Boar Semen.Animals : an open access journal from MDPI · 2025Article
- Effects of cactus (Animal reproduction · 2025Article
- Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin.Journal of animal science and biotechnology · 2024Article
- Palm Kernel Meal Protein Hydrolysates Enhance Post-Thawed Boar Sperm Quality.Animals : an open access journal from MDPI · 2023Article
- The Beneficial Effect of Resveratrol on the Quality of Frozen-Thawed Boar Sperm.Animals : an open access journal from MDPI · 2023Article
- Antioxidant Effects of Myo-Inositol Improve the Function and Fertility of Cryopreserved Boar Semen.Antioxidants (Basel, Switzerland) · 2023Article
- Pharmacological Evidence Suggests That Slo3 Channel Is the Principal KInternational journal of molecular sciences · 2023Article
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Authors and funding
14 authors.
Funding
Abstract
Reactive oxygen species (ROS) produced during freeze−thaw procedures cause oxidative damage to the sperm, reducing fertility. We aimed to improve the post-thaw quality of pig sperm by quercetin (QRN) supplementation to reduce the cryodamage associated with the freeze−thaw procedure. Four equal aliquots of pooled boar semen were diluted with a freezing extender supplemented with different concentrations of QRN (0, 25, 50, and 100 µM) and then were subjected to cryopreservation in liquid nitrogen. Semen analysis was performed following 7 days of cryopreservation. Results demonstrated that the semen samples supplemented with 50 µM QRN significantly improved the post-thaw sperm quality than those subjected to other supplementations (p < 0.05). Semen samples supplemented with 50 µM QRN showed significantly improved plasma membrane functional integrity (47.5 ± 1.4 vs. 43.1 ± 4.1, 45.3 ± 1.7, and 44.1 ± 1.4) and acrosome integrity (73.6 ± 3.4 vs. 66.3 ± 2.4, 66.7 ± 3.6, and 68.3 ± 32.9) as compared to the control, 25 µM, and 100 µM QRN groups, respectively. The mitochondrial activity of the 50 µM QRN group was greater than control and 25 µM QRN groups (43.0 ± 1.0 vs. 39.1 ± 0.9 and 41.9 ± 1.0) but showed no difference with the 100 µM QRN group. Moreover, the 50 µM QRN group showed a higher sperm number displaced to 1 cm and 3 cm points in the artificial mucus than other groups. Therefore, supplementing the freezing extender with QRN can serve as an effective tool to reduce the magnitude of oxidative damage associated with sperm freezing.
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